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Esterase EstE from Xanthomonas vesicatoria (Xv_EstE) is an outer membrane protein capable of hydrolyzing long-chain polar esters

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Abstract.

A new esterase gene from Xanthomonas vesicatoria (formerly X. campestris) DSM 50861 was identified, cloned from a chromosomal gene library and overexpressed in Escherichia coli. The corresponding DNA fragment contains an ORF of 1,818 bp, encoding a hydrolase of the GDSL esterase family. A protein of about 67 kDa, named Xv_EstE, was expressed from this fragment. A N-terminal signal peptide was processed under low-expression conditions, yielding a 63-kDa mature protein. The predicted amino acid sequence showed distinct homology to esterases of the GDSL family. Based on homology, a catalytic triad Gly-Asp-Ser could be defined. Amino acid sequence alignments and computer-assisted structure prediction indicated the presence of a carboxyl-terminal β-barrel membrane domain which might facilitate binding of Xv_EstE to the outer membrane. This could be verified by differential cell fractionation experiments, in which Xv_EstE was exclusively found in the outer membrane fraction. Xv_EstE showed preferential hydrolytic activity on short chain (up to C8) and para-substituted nitrophenylesters as substrates. However, only long-chain 1-hydroxy-pyrene-3,6,8-trisulfonic acid (HPTS)-fatty acid esters were hydrolyzed. Xv_EstE was also found to be active on a series of substrates of industrial interest, such as 1-methylprop-2-ynyl acetate, for which an enantioselectivity up to 93% ee could be recognized.

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Acknowledgements

This work was supported by the Austrian FWF, SFB Biocatalysis, project number F001 0101. We thank Dr. Erich Lanka for providing us with plasmid pMS470Δ8 and Martina Palzer and Gerit Schmidt-Polaschek for excellent technical assistance.

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Authors and Affiliations

  1. Institut für Biotechnologie, Arbeitsgruppe Genetik, Technische Universitaet Graz, Petersgasse 12, 8010, Graz, Austria

    D. Talker-Huiber, A. Glieder, M. Pressnig, G. Stubenrauch & H. Schwab

  2. Pharmazeutische und Medizinische Chemie, Universitaet des Saarlandes, Postfach 151150, 66041, Saarbruecken, Germany

    J. Jose

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  1. D. Talker-Huiber
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  2. J. Jose
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  3. A. Glieder
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  5. G. Stubenrauch
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  6. H. Schwab
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Correspondence to H. Schwab.

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Talker-Huiber, D., Jose, J., Glieder, A. et al. Esterase EstE from Xanthomonas vesicatoria (Xv_EstE) is an outer membrane protein capable of hydrolyzing long-chain polar esters. Appl Microbiol Biotechnol 61, 479–487 (2003). https://doi.org/10.1007/s00253-003-1227-5

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