Skip to main content

Advertisement

SpringerLink
Log in

Cloning and chromosomal mapping of an orphan chemokine receptor: mouse RDC1

  • ORIGINAL PAPER
  • Published:
Immunogenetics Aims and scope Submit manuscript

Abstract

 Degenerate RT-PCR was used to identify a new seven-transmembrane-spanning receptor expressed in astrocytes. A receptor, termed RDC1, displaying the characteristic structural features of a chemokine receptor was cloned. The predicted 362-amino-acid sequence displayed 92% and 91% similarity to the human and dog orphan receptor RDC1, respectively. In addition, RDC1 shares 43% amino acid similarity to rabbit and mouse CXCR2. Transcripts of RDC1 were found in astrocytes, heart, kidney, the mesangial tumor line MES-13, spleen, and neutrophils by means of northern blot. Using linkage analysis of interspecies backcross mice, we localized to chromosome 1 the genes for mouse CXCR2, CXCR4, and RDC1. Mouse RDC1 is linked to and lies between the genes for the mouse CXC chemokine receptors CXCR2 and CXCR4. The combined data of chromosomal location and sequence similarity suggest that RDC1 is an orphan CXC chemokine receptor.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Author information

Authors and Affiliations

  1. Department of Pathology, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115, USA, , , , , , US

    Michael Heesen, Michael A. Berman & M. E. Dorf

  2. Center for Cancer Research, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA, , , , , , US

    Alain Charest & David Housman

  3. Ina Sue Perlmutter Research Laboratories, Children’s Hospital, 230 Longwood Avenue, Boston, MA 02115, USA, , , , , , US

    Craig Gerard

Authors
  1. Michael Heesen
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Michael A. Berman
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Alain Charest
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. David Housman
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Craig Gerard
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. M. E. Dorf
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Received: 10 July 1997 / Revised: 6 October 1997

Rights and permissions

Reprints and permissions

About this article

Cite this article

Heesen, M., Berman, M., Charest, A. et al. Cloning and chromosomal mapping of an orphan chemokine receptor: mouse RDC1. Immunogenetics 47, 364–370 (1998). https://doi.org/10.1007/s002510050371

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/s002510050371

Search

Navigation