Abstract
High-mobility group box protein 1 (HMGB1) gene has a universal sentinel function for nucleic acid-mediated innate immune responses and acts as a pathogenic mediator in the inflammatory disease. In an effort to identify the functional single-nucleotide polymorphism (SNP) in the 3′-untranslated region (UTR) of the bovine HMGB1 gene that affects the binding to its target microRNA, first, the expression of HMGB1 mRNA in different genotypes and its candidate bta-miR-223 was investigated. Quantitative real-time polymerase chain reaction results showed that the relative expression of HMGB1 mRNA in cows with the genotype GG is significantly higher than those in cows with the genotype AA (P < 0.05). The expression of bta-miR-223 was significantly upregulated by 1.95-fold (P < 0.05) in the bovine mastitis-infected mammary gland tissues compared with that in the healthy tissues. Subsequently, luciferase assay indicated that the HMGB1 expression was directly targeted by bta-miR-223 in human embryo kidney 293 T (HEK 293T) cells. One novel SNP (g. +2776 A > G) in the HMGB1 3′-UTR, altering the binding of HMGB1 and bta-miR-223, was found to be associated with somatic count scores in cows. Taken together, the g. +2776 A > G-GG was an advantageous genotype which can be used as a candidate functional marker for mastitis resistance breeding program.
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Acknowledgments
This study was supported by grants from the National Natural Science Foundation of China (no. 31000543), Major Project of National Transgene in China (2011ZX08007-001), Support Program of the Ministry of Science and Technology, People’s Republic of China (2011BAD19B02, 2011BAD19B04), China Agriculture Research System (CARS-37), Project of Agricultural Fine Breed from the Department of Science and Technology of Shandong Province (2010LZ10-02).
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Fig. S1
Identification of the pMIR-HMGB1 by restriction PCR and enzyme digestion. M, DL2000 DNA marker; 1, 2, PCR products of plasmid with the different alleles at g. +2776; 3, 4, restriction enzyme digestion products of the different plasmids (DOC 58 kb)
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Li, L., Huang, J., Zhang, X. et al. One SNP in the 3′-UTR of HMGB1 gene affects the binding of target bta-miR-223 and is involved in mastitis in dairy cattle. Immunogenetics 64, 817–824 (2012). https://doi.org/10.1007/s00251-012-0641-1
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DOI: https://doi.org/10.1007/s00251-012-0641-1