Abstract
Recent large-scale cDNA cloning studies have shown that a significant proportion of the transcripts expressed from vertebrate genomes do not appear to encode protein. Moreover, it was reported in mammals (human and mice) that these non-coding transcripts are expressed and regulated by mechanisms similar to those involved in the control of protein-coding genes. We have produced a collection of cDNA sequences from immunologically active tissues with the aim of discovering chicken genes involved in immune mechanisms, and we decided to explore the non-coding component of these immune-related libraries. After finding known non-coding RNAs (miRNA, snRNA, snoRNA), we identified new putative mRNA-like non-coding RNAs. We characterised their expression profiles in immune-related samples. Some of them showed changes in expression following viral infections. As they exhibit patterns of expression that parallel the behaviour of protein-coding RNAs in immune tissues, our study suggests that they could play an active role in the immune response.
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Acknowledgements
This research was supported by funds from the European Commission to RZ (KA5-QLRT-CT99-1591 Chicken IMAGE). T.R. was supported by grants from the SABRE Integrated European project (FOOD-CT-2006-016250), and M.E.A. by grants from the French Ministere de la Recherche. We thank Dr F. Dautry for helpful discussion.
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Additional data file 1
Table of putative ncRNAs associated data including localisation, comparison with previous study and expression information (XLS 52 kb)
Additional data file 2
Dendrograms of the 15 clusters obtained by k-mean clustering of both coding and non-coding transcripts based on expression levels from immune-related tissues (GIF 5841 kb)
Additional data file 3
Descriptions of the transcripts included in each expression cluster of the immune-related tissues analysis (TXT 138 kb)
Additional data file 4
Dendrograms of the 15 clusters obtained by k-mean clustering of both coding and non-coding transcripts based on expression levels from the IBDV infection (GIF 2032 kb)
Additional data file 5
Descriptions of the transcripts included in each expression cluster of the IBDV infection analysis (TXT 114 kb)
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Ahanda, ML.E., Ruby, T., Wittzell, H. et al. Non-coding RNAs revealed during identification of genes involved in chicken immune responses. Immunogenetics 61, 55–70 (2009). https://doi.org/10.1007/s00251-008-0337-8
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DOI: https://doi.org/10.1007/s00251-008-0337-8