Abstract
Genes encoding the immunoglobulin heavy-chain variable region (Ig VH) in rainbow trout (Oncorhynchus mykiss) have been grouped into 11 families. While obtaining a baseline assessment of the various gene families utilized by trout in the production of secreted antibody, we discovered two new families. These proposed Ig VH families, Families XII and XIII, were rarely observed; only two VH sequence types were detected for each new family, suggesting that they may not be commonly used in response to antigens, or that the captive environment may not lead to typical exposures seen in the wild. Additionally, unlike preceding studies, we found at least one representative gene sequence for each of the 11 reported Ig VH gene families, possibly indicating that the repertoire of trout Ig VH gene families may be more universal among different stocks than previously realized.
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References
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Acknowledgments
This project was supported by National Research Initiative Grant no. 2004-35205-14199 from the USDA Cooperative State Research, Education, and Extension Service. The authors also wish to thank Dr. G. Wiens (USDA, C.C.C.C.W.A.) for the provision of the trout used in this study and Dr. E. Bromage, Dr. P. Zwollo, Dr. K. Reece, Ms. M. Vogelbein, Ms. C. Spier, Ms. S. MacKinley, and Mr. J. Ye for their critical reviews of the manuscript. VIMS #2227
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GenBank accession numbers: Family XII—DQ453185 and DQ453150; Family XIII—DQ453153 and DQ453146; others DQ453143, DQ453156, DQ831723, DQ831825.
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Brown, G.D., Kaattari, I.M. & Kaattari, S.L. Two new Ig VH gene families in Oncorhynchus mykiss . Immunogenetics 58, 933–936 (2006). https://doi.org/10.1007/s00251-006-0149-7
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DOI: https://doi.org/10.1007/s00251-006-0149-7