Abstract
The T-cell-specific adapter protein (TSAd), encoded by the SH2D2A gene, has been implicated in modulation of proximal signaling events as well as in transcriptional regulation in human T cells. We have isolated its rat homologue (rSH2D2A) from an NK cell cDNA library and mapped the corresponding gene to chromosome 2 with a hamster-rat radiation hybrid cell panel. rSH2D2A encodes a 376 amino acid protein (rTSAd) which shows greater homology to mouse than human TSAd. In rats, rTSAd was specifically expressed by NK cells and T cells but not by other leukocytes tested. Similarly, in humans we observed abundant transcripts for TSAd in NK cells and T cells. The data suggest that TSAd may have a regulatory role in cellular activation of T and NK cells.
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Acknowledgements
This study was supported by the Norwegian Cancer Society and the Norwegian Research Council. The technical assistance of Lise Berven and Ingebjørg Knutsen Heitman is acknowledged.
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Nucleotide sequence data reported are available in the GenBank database under the accession number: NM_207605.
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Nejad, S., Bryceson, Y., Dissen, E. et al. cDNA cloning of a rat orthologue of SH2D2A encoding T-cell-specific adaptor protein (TSAd): expression in T and NK cells. Immunogenetics 56, 338–342 (2004). https://doi.org/10.1007/s00251-004-0695-9
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DOI: https://doi.org/10.1007/s00251-004-0695-9