Abstract
Monotremes are an ancient mammalian lineage that last shared a common ancestor with the marsupial and eutherian (placental) mammals about 170 million years ago. Characterization of their immune genes is allowing us to gain insights into the evolutionary processes that lead to the ‘mammalian’ immune response. Here we describe the characterization of the first cDNA clones encoding T-cell receptors from a monotreme. Two TCR α-chain cDNAs (TCRA) from the short-beaked echidna, Tachyglossus aculeatus, containing complete variable, joining and constant regions were isolated. The echidna TCRA constant region shares approximately 37% amino acid identity with other mammalian TCRA constant region sequences. The two variable regions belong to the TCRAV group C, which also contains V genes from humans, mice, cattle and chickens. One echidna TCR β-chain cDNA (TCRB) containing the entire constant region was isolated and sequenced. It shares about 63% identity with other mammalian TCRB constant region sequences. The echidna TCRBV belongs to TCRBV group A, which also contains V genes from various eutherian species. Southern blot analysis indicates that, like in other mammalian species, there is only one TCRA constant region copy in the echidna genome, but at least two TCRB constant regions.
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References
Alcover A, Mariuzza RA, Ermonval M, Acuto O (1990) Lysine 271 in the transmembrane domain of the T-cell antigen receptor beta chain is necessary for its assembly with the CD3 complex but not for alpha/beta dimerization. J Biol Chem 265:4131–4135
Baker ML, Rosenberg GH, Zuccolotto P, Harrison GA, Deane EM, Miller RD (2001) Further characterization of T cell receptor chains of marsupials. Dev Comp Immunol 25:495–507
Belov K, Hellman L, Cooper DW (2002) Characterisation of IgG1 from a monotreme, Tachyglossus aculeatus. Immunogenetics 53:1065–1071
Belov K, Lam MK, Hellman L, Colgan DJ (2003) Evolution of the major histocompatibility complex: isolation of class II β cDNAs from two monotremes, the platypus and the short-beaked echidna. Immunogenetics 55:402–411
Campbell KS, Backstrom BT, Tiefenthaler G, Palmer E (1994) CART: a conserved antigen receptor transmembrane motif. Semin Immunol 6:393–410
Charlemagne J, Fellah JS, De Guerra A, Kerfourn F, Partula S (1998) T-cell receptors in ectothermic vertebrates. Immunol Rev 166:87–102
Chothia C, Boswell DR, Lesk AM (1988) The outline structure of the T-cell alpha beta receptor. EMBO J 7:3745–3755
Connolly JH, Canfield PJ, McClure SJ, Whittington RJ (1999) Histological and immunohistological investigation of lymphoid tissue in the platypus (Ornithorhynchus anatinus). J Anat 195:161–171
Garcia KC, Degano M, Stanfield RL, Brunmark A, Jackson MR, Peterson PA, Teyton L, Wilson IA (1996) An alphabeta T cell receptor structure at 2.5 A and its orientation in the TCR-MHC complex. Science 274:209–219
Harrison GA, Taylor CL, Miller RD, Deane EM (2003) Primary structure and variation of the T-cell receptor delta-chain from a marsupial, Macropus eugenii. Immunol Lett 88:117–125
Kumar S, Tamura K, Jakobsen IB, Nei M (2001) MEGA2: molecular evolutionary genetics analysis software. Bioinformatics 17:1244–1245
Li ZG, Wu WP, Manolios N (1996) Structural mutations in the constant region of the T-cell antigen receptor (TCR) beta chain and their effect on TCR alpha and beta chain interaction. Immunology 88:524–530
Livak F, Schatz DG (1998) Alternative splicing of rearranged T cell receptor delta sequences to the constant region of the alpha locus. Proc Natl Acad Sci USA 95:5694–5699
Miska KB, Harrison GA, Hellman L, Miller RD (2002) The major histocompatibility complex in monotremes: an analysis of the evolution of Mhc class I genes across all three mammalian subclasses. Immunogenetics 54:381–393
Miska KB, Hellman L, Miller RD (2003) Characterization of B2-microglobulin coding sequence from three non-placental mammals: the duckbill platypus, the short-beaked echidna, and the grey short-tailed opossum. Dev Comp Immunol 27:247–256
Musser AM (2003) Review of the monotreme fossil record and comparison of palaeontological and molecular data. Comp Biochem Physiol A 136:927–942
Saito H, Kranz DM, Takagaki Y, Hayday AC, Eisen HN, Tonegawa S (1984) Complete primary structure of a heterodimeric T-cell receptor deduced from cDNA sequences. Nature 309:757–762
Su C, Jakobsen I, Gu X, Nei M (1999) Diversity and evolution of T-cell receptor variable region genes in mammals and birds. Immunogenetics 50:301–308
Thompson JD, Higgins DG, Gibson TJ (1994) CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680
Zuccolotto PD, Harrison GA, Deane EM (2000) Cloning of marsupial T cell receptor alpha and beta constant region cDNAs. Immunol Cell Biol 78:103–109
Acknowledgements
This work was supported by an Australian Research Council Discovery Grant to KB and a US National Science Foundation award MCB-9981960 to RDM.
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Belov, K., Miller, R.D., Ilijeski, A. et al. Isolation of monotreme T-cell receptor α and β chains. Immunogenetics 56, 164–169 (2004). https://doi.org/10.1007/s00251-004-0679-9
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DOI: https://doi.org/10.1007/s00251-004-0679-9