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Analysis of genomic and expressed major histocompatibility class Ia and class II genes in a hexaploid Lake Tana African ‘large’ barb individual (Barbus intermedius)

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Abstract

Expression of too many co-dominant major histocompatibility complex (MHC) alleles is thought to be detrimental to proper functioning of the immune system. Polyploidy of the genome will increase the number of expressed MHC genes unless they are prone to a silencing mechanism. In polyploid Xenopus species, the number of MHC class I and II genes has been physically reduced, as it does not increase with higher ploidy genomes. In the zebrafish some class IIB loci have been silenced, as only two genomically bona fide loci, DAA/DAB and DEA/DEB, have been described. Earlier studies indicated a reduction in the number of genomic and expressed class II MHC genes in a hexaploid African ‘large’ barb. This prompted us to study the number of MHC genes present in the genome of an African ‘large’ barb individual (Barbus intermedius) in relation to those expressed, adopting the following strategy. Full-length cDNA sequences were generated from mRNA and compared with partial genomic class Ia and II sequences generated by PCR using the same primer set. In addition, we performed Southern hybridizations to obtain a verification of the number of class I and IIB genes. Our study revealed three β2-microglobulin, five class Ia, four class IIA, and four class IIB genes at the genomic level, which were shown to be expressed in the hexaploid barb individual. The class Ia and class II data indicate that the ploidy status does not correlate with the presence and expression of these MHC genes.

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Acknowledgements

The authors would like to thank Martin de Graaf, Leo Nagelkerke and Nand Sibbing for providing tissue samples of a Lake Tana Barbus intermedius individual.

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Authors and Affiliations

  1. Cell Biology and Immunology Group, Wageningen Institute of Animal Sciences, Wageningen University, P.O. Box 338, 6700 AH, Wageningen, The Netherlands

    Corine P. Kruiswijk, Trudi Hermsen, Huub F. J. Savelkoul & René J. M. Stet

  2. Department of Biology, University of Waterloo, Waterloo, Ontario, Canada

    Kazuhiro Fujiki & Brian Dixon

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  1. Corine P. Kruiswijk
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  2. Trudi Hermsen
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  3. Kazuhiro Fujiki
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  4. Brian Dixon
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  5. Huub F. J. Savelkoul
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  6. René J. M. Stet
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Correspondence to René J. M. Stet.

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Kruiswijk, C.P., Hermsen, T., Fujiki, K. et al. Analysis of genomic and expressed major histocompatibility class Ia and class II genes in a hexaploid Lake Tana African ‘large’ barb individual (Barbus intermedius). Immunogenetics 55, 770–781 (2004). https://doi.org/10.1007/s00251-003-0635-0

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  • DOI: https://doi.org/10.1007/s00251-003-0635-0

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