Abstract
In order to identify and characterize genetic polymorphism of the swine major histocompatibility complex (Mhc: SLA) class I genes, RT-PCR products of the second and third exons of the three SLA classical class I genes, SLA-1, SLA-2 and SLA-3 were subjected to nucleotide determination. These analyses allowed the identification of four, eight and seven alleles at the SLA-1, SLA-2 and SLA-3 loci, respectively, from three different breeds of miniature swine and one mixed breed. Among them, 12 alleles were novel. Construction of a phylogenetic tree using the nucleotide sequences of those 19 alleles indicated that the SLA-1 and -2 genes are more closely related to each other than to SLA-3. Selective forces operating at single amino acid sites of the SLA class I molecules were analyzed by the Adaptsite Package program. Ten positive selection sites were found at the putative antigen recognition sites (ARSs). Among the 14 positively selected sites observed in the human MHC (HLA) classical class I molecules, eight corresponding positions in the SLA class I molecules were inferred as positively selected. On the other hand, four amino acids at the putative ARSs were identified as negatively selected in the SLA class I molecules. These results suggest that selective forces operating in the SLA class I molecules are almost similar to those of the HLA class I molecules, although several functional sites for antigen and cytotoxic T-lymphocyte recognition by the SLA class I molecules may be different from those of the HLA class I molecules.
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Acknowledgements
We would like to thank Dr. Patrick Chardon (INRA-CEA, France) for useful discussion. We also wish to thank Dr. Makiko Shiba and Mariko Tsustui (Ibaraki Station, National Livestock Breeding Center, Independent Administrative Institution) for providing the peripheral blood samples from Mexican hairless pigs, and Mr. Daisuke Sumiyama (Tokai University School of Medicine) for technical assistance. This study was supported by grants from the Ministry of Education, Culture, Sports, Science and Technology, Japan, and the Animal Genome Research Project of the Ministry of Agriculture, Forestry and Fisheries of Japan.
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The DNA sequence data reported in this paper have been submitted to the DDBJ, EMBL and GenBank nucleotide databases and have been assigned the accession numbers, AB105379, AB105380, AB105381, AB105382, AB105383, AB105384, AB105385, AB105386, AB105388, AB105389, AB105390 and AB105391
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Ando, A., Kawata, H., Shigenari, A. et al. Genetic polymorphism of the swine major histocompatibility complex (SLA) class I genes, SLA-1, -2 and -3 . Immunogenetics 55, 583–593 (2003). https://doi.org/10.1007/s00251-003-0619-0
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DOI: https://doi.org/10.1007/s00251-003-0619-0