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Molecular characterization of major histocompatibility complex class 1 (MHC-I) from squirrel monkeys (Saimiri sciureus)

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Abstract

Little is known about the major histocompatibility complex (MHC) class 1 in squirrel monkeys (Saimiri sciureus). We cloned, sequenced and characterized two alleles and the cDNA of the coding region of MHC class 1 in these New World monkeys. Phylogenetic analyses showed that these sequences are related to HLA class 1 genes (HLA-A and HLA-G). The structure and organization of one of the two identified clones was similar to that of a class 1 MHC gene (HLA-A2). All the exon/intron splice acceptor/donor sites are conserved and their locations correspond to the HLA-A2 gene. The sequences of the newly described cDNAs reveal that they code for the characteristic class 1 MHC proteins, with all the features thought necessary for cell surface expression. Typical sequences for the leader peptide, α1, α2, α3, transmembrane and cytoplasmic domains were found.

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Acknowledgements

We thank J.F. Pouliquen for technical assistance. We also thank the Ministry of Research (Programme de Recherche fondamentale en Microbiologie des Maladies Infectieuses et Parasitaires), the Association pour la Recherche contre le Cancer (ARC) and the Virus Cancer Prevention (VCP) association for financial support.

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Authors and Affiliations

  1. Laboratoire de Rétrovirologie, Institut Pasteur de la Guyane, 23 avenue Pasteur, BP 6010, 97306 , Cayenne, French Guiana

    Hervé Pascalis, Jean-Michel Heraud, Rolf Fendel & Mirdad Kazanji

  2. Centre de Primatologie, Institut Pasteur de la Guyane, 97306, Cayenne, French Guiana

    Anne Lavergne

Authors
  1. Hervé Pascalis
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  2. Jean-Michel Heraud
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  3. Rolf Fendel
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  4. Anne Lavergne
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  5. Mirdad Kazanji
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Corresponding author

Correspondence to Mirdad Kazanji.

Additional information

The nucleotide sequence data reported in this paper have been submitted to the GenBank database and have been assigned the accession numbers AJ438576 (Sasc-G*31), AJ438577 (Sasc-G*25), AY282760 (Sasc-G*03), AY282761 (Sasc-G*04) and AY282762 (Sasc-G*05). Sequences were named as recommended by Klein and co-workers (1990)

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Pascalis, H., Heraud, JM., Fendel, R. et al. Molecular characterization of major histocompatibility complex class 1 (MHC-I) from squirrel monkeys (Saimiri sciureus). Immunogenetics 55, 633–639 (2003). https://doi.org/10.1007/s00251-003-0607-4

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  • DOI: https://doi.org/10.1007/s00251-003-0607-4

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