Abstract
The mathematical expression of the signal to noise ratio in fluorescence fluctuation experiments is derived for arbitrary sample profiles and for any mechanism of translational motion, and experimentally verified. The signal to noise ratio depends on the mean count rate per particle per dwell time, the mean number of particles per sample volume, time characteristics of the correlation function, sample profile characteristics, and the data collection time. Statistical accuracy of the third order moment of fluorescence intensity fluctuations is also studied. The optimum concentration for the third order moment analysis is about one particle per sample volume.
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Received: 13 February 1996 / Accepted: 20 September 1996
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Kask, P., Günther, R. & Axhausen, P. Statistical accuracy in fluorescence fluctuation experiments. Eur Biophys J 25, 163–169 (1997). https://doi.org/10.1007/s002490050028
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DOI: https://doi.org/10.1007/s002490050028