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Quantification of the interceptor action of caffeine on the in vitro biological effect of the anti-tumour agent topotecan

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Abstract

Using published in vitro data on the dependence of the percentage of apoptosis induced by the anti-cancer drug topotecan in a leukaemia cell line on the concentration of added caffeine, and a general model of competitive binding in a system containing two aromatic drugs and DNA, it has been shown to be possible to quantify the relative change in the biological effect just using a set of component concentrations and equilibrium constants of the complexation of the drugs. It is also proposed that a general model of competitive binding and parameterization of that model may potentially be applied to any system of DNA-targeting aromatic drugs under in vitro conditions. The main reasons underpinning the proposal are the general feature of the complexation of aromatic drugs with DNA and their interaction in physiological media via hetero-association.

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Acknowledgments

Prof. David Gadian (ICH) is thanked for use of the NMR spectrometer.

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Authors and Affiliations

  1. Department of Physics, Sevastopol National Technical University, Universitetskaya str., 33, Sevastopol, 99053, Ukraine

    M. P. Evstigneev, A. A. Mosunov & V. P. Evstigneev

  2. Institute of Cancer Research, University of London, Sutton, UK

    H. G. Parkes

  3. School of Biological and Chemical Sciences, Birkbeck College, University of London, London, UK

    D. B. Davies

Authors
  1. M. P. Evstigneev
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  2. A. A. Mosunov
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  3. V. P. Evstigneev
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  4. H. G. Parkes
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  5. D. B. Davies
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Corresponding author

Correspondence to M. P. Evstigneev.

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Evstigneev, M.P., Mosunov, A.A., Evstigneev, V.P. et al. Quantification of the interceptor action of caffeine on the in vitro biological effect of the anti-tumour agent topotecan. Eur Biophys J 40, 969–980 (2011). https://doi.org/10.1007/s00249-011-0712-5

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  • DOI: https://doi.org/10.1007/s00249-011-0712-5

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