Abstract
Proteo-giant liposomes were electroformed from a mixture of lecithin vesicles and inside-out vesicles from erythrocytes. After addition of Mg-ATP in the vicinity of the proteo-giant liposomes, small buds appeared on the liposome surfaces, which—via an increase in lipids in the outer monolayer—demonstrated the active transport of lipids from the inner to the outer monolayer, indicating flippase activity.
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References
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Acknowledgments
The authors thank Dr. Bertrand (INSERM U665, Paris) for his continuous help through out this work. We thank also Dr. Ivan López-Montero for useful discussions. This work was supported by grants from the Université Paris 7-Denis Diderot, from the Centre National de la Recherche Scientifique, Unité mixte de recherche 7099, from the Research Training Network on Phospholipid Flippases from the European Community MRTNCT-2004-005330, and from the Agence Nationale de la Recherche 2003-2-313.
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Ezanno, P., Cribier, S. & Devaux, P.F. Asymmetrical stress generated by the erythrocyte lipid flippase triggers multiple bud formation on the surface of spherical giant liposomes. Eur Biophys J 39, 1277–1280 (2010). https://doi.org/10.1007/s00249-009-0557-3
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DOI: https://doi.org/10.1007/s00249-009-0557-3