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Cholesterol modulation of nicotinic acetylcholine receptor surface mobility

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Abstract

Nicotinic acetylcholine receptor (AChR) function and distribution are quite sensitive to cholesterol (Chol) levels in the plasma membrane (reviewed by Barrantes in J Neurochem 103 (suppl 1):72–80, 2007). Here we combined confocal fluorescence recovery after photobleaching (FRAP) and confocal fluorescence correlation spectroscopy (FCS) to examine the mobility of the AChR and its dependence on Chol content at the cell surface of a mammalian cell line. Plasma membrane AChR exhibited limited mobility and only ~55% of the fluorescence was recovered within 10 min after photobleaching. Depletion of membrane Chol by methyl-β-cyclodextrin strongly affected the mobility of the AChR at the plasma membrane; the fraction of mobile AChR fell from 55 to 20% in Chol-depleted cells, whereas Chol enrichment by methyl-β-cyclodextrin-Chol treatment did not reduce receptor mobility at the cell surface. Actin depolymerization caused by latrunculin A partially restored receptor mobility in Chol-depleted cells. In agreement with the FRAP data, scanning FCS experiments showed that the diffusion coefficient of the AChR was about 30% lower upon Chol depletion. Taken together, these results suggest that membrane Chol modulates AChR mobility at the plasma membrane through a Chol-dependent mechanism sensitive to cortical actin.

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Abbreviations

αBTX:

αBungarotoxin

AChR:

Nicotinic acetylcholine receptor

BODIPY-FL-C5-SM:

N-(4,4-difluoro-5, 7-dimethyl-4- bora-3a, 4a-diazo-s-indacene-3-pentanoyl)sphingosyl phosphocholine

CDx:

Methyl-β-cyclodextrin

Chol:

Cholesterol

CDx–Chol:

Methyl-β-cyclodextrin–cholesterol

FCS:

Fluorescence correlation spectroscopy

FRAP:

Fluorescence recovery after photobleaching

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Acknowledgments

Thanks are due to Professor Toshihide Kobayashi and Dr Satoshi B. Sato, of the Lipid Biology Laboratory, RIKEN Institute of Physical and Chemical Research, Discovery Research Institute, Saitama, and Department of Biophysics, Kyoto University, Japan, for providing a sample of f-PEG-Chol, and to Dr Jorge J. Wenz and Ms Virginia Borroni for helpful discussion. Research described in this article was supported in part by PICT 01-12790 and 5-20155 from FONCYT, Ministry of Science and Technology; PIP No. 6367 from the Argentinian Scientific Research Council (CONICET); Philip Morris USA Inc., and Philip Morris International; and PGI No. 24/B135 from Universidad Nacional del Sur, Argentina, to F.J.B.

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  1. UNESCO Chair of Biophysics and Molecular Neurobiology, Instituto de Investigaciones Bioquímicas de Bahía Blanca, C.C. 857, B8000FWB, Bahía Blanca, Argentina

    Carlos J. Baier, Cristina E. Gallegos & Francisco J. Barrantes

  2. Laboratorio de Electrónica Cuántica, Departamento de Física, Universidad de Buenos Aires, 1428, Buenos Aires, Argentina

    Valeria Levi

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  1. Carlos J. Baier
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  2. Cristina E. Gallegos
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  3. Valeria Levi
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  4. Francisco J. Barrantes
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Correspondence to Francisco J. Barrantes.

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Baier, C.J., Gallegos, C.E., Levi, V. et al. Cholesterol modulation of nicotinic acetylcholine receptor surface mobility. Eur Biophys J 39, 213–227 (2010). https://doi.org/10.1007/s00249-009-0521-2

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