Abstract
Sedimentation velocity experiments can be used to identify two or more independent non-interacting macromolecules, which differ in their size by only a few percent. The procedure requires the extrapolation of differential apparent sedimentation coefficient distributions obtained at different running time to t → ∞ and works because it eliminates or greatly reduces diffusion effects. Here, we present an improved time extrapolation function of sedimentation distribution profiles originally presented by Stafford (In: Harding, Rowe, Horton (eds.) Analytical ultracentrifugation in biochemistry and polymer science, 1992). We describe a computing procedure with the program lamm to analyze concentration profiles obtained by absorbance or interference optics that utilizes suitable smoothing methods for noisy data sets and present examples which include time invariant noises.
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AUC&HYDRO 2008—Contributions from 17th International Symposium on Analytical Ultracentrifugation and Hydrodynamics, Newcastle, UK, 11–12 September 2008.
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Behlke, J., Ristau, O. Enhanced resolution of sedimentation coefficient distribution profiles by extrapolation to infinite time. Eur Biophys J 39, 449–455 (2010). https://doi.org/10.1007/s00249-009-0425-1
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DOI: https://doi.org/10.1007/s00249-009-0425-1