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Functional membrane diffusion of G-protein coupled receptors

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Abstract

G-protein-coupled receptor function involves interactions between the receptor, G-proteins and effectors in the cell plasma membrane. The main biochemical processes have been individually identified but the mechanisms governing the successive protein–protein interactions of this complex multi-molecular machinery have yet to be established. We discuss advances in understanding the functional dynamics of the receptor resulting from diffusion measurements, and in the context of the plasma membrane organization.

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Abbreviations

GPCR:

G-protein-coupled receptor

FRAP:

Fluorescence recovery after photobleaching

vrFRAP:

Variable radius FRAP

SPT:

Single particle tracking

SMT:

Single molecule tracking

FCS:

Fluorescence correlation spectroscopy

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Acknowledgments

The authors would like to thank F. Daumas and F. Viala for their help in editing the figures. We apologize in advance to the authors whose work we have omitted.

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Authors and Affiliations

  1. Institut de Pharmacologie et Biologie Structurale, UMR CNRS-Université Paul Sabatier 5089, 205, route de Narbonne, 31077, Toulouse cedex 09, France

    Aurélie Baker, Aude Saulière, Fabrice Dumas, Claire Millot, Serge Mazères, André Lopez & Laurence Salomé

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  1. Aurélie Baker
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  2. Aude Saulière
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  3. Fabrice Dumas
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  4. Claire Millot
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  5. Serge Mazères
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  6. André Lopez
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  7. Laurence Salomé
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Corresponding author

Correspondence to Laurence Salomé.

Additional information

Aurélie Baker and Aude Saulière contributed equally to this work.

Presented at the joint biannual meeting of the SFB-GEIMM-GRIP, Anglet France, 14–19 October, 2006.

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Baker, A., Saulière, A., Dumas, F. et al. Functional membrane diffusion of G-protein coupled receptors. Eur Biophys J 36, 849–860 (2007). https://doi.org/10.1007/s00249-007-0214-7

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  • DOI: https://doi.org/10.1007/s00249-007-0214-7

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