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Sedimentation equilibrium: a valuable tool to study homologous and heterogeneous interactions of proteins or proteins and nucleic acids

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Abstract

We present a short overview of our experience in analyzing the affinity and stoichiometry of self-associating and heterologous interactions by using the sedimentation equilibrium technique. Data acquisition and the fitting procedure employing the computer programs that we have developed, Polymole and Virial, are utilized for obtaining reliable results under ideal as well as non-ideal conditions. Such data derived from biologically important macromolecules find utility in understanding physiological events such as cell regulation.

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Acknowledgements

Many of the experiments presented here were carried out in fruitful collaboration with Thomas Kriegel, Dresden; Sabine Brantl, Jena; Heinz Welfle, Berlin; Udo Heinemann, Berlin; Hartmut Oschkinat, Berlin; Alexander Rich, Cambridge, Mass.; Hannelore Haase, Berlin. We are grateful to all these colleagues and thank Howard Etlinger for critical reading the manuscript. The programs Polymole and Virial are available on request.

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Authors and Affiliations

  1. Max Delbrück Center for Molecular Medicine, Robert Rössle Strasse 10, 13092 , Berlin, Germany

    Joachim Behlke & Otto Ristau

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  1. Joachim Behlke
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  2. Otto Ristau
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Correspondence to Joachim Behlke.

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Presented at the conference for Advances in Analytical Ultracentrifugation and Hydrodynamics, 8–11 June 2002, Grenoble, France

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Behlke, J., Ristau, O. Sedimentation equilibrium: a valuable tool to study homologous and heterogeneous interactions of proteins or proteins and nucleic acids. Eur Biophys J 32, 427–431 (2003). https://doi.org/10.1007/s00249-003-0318-7

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  • DOI: https://doi.org/10.1007/s00249-003-0318-7

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