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Diversity and Persistence of Ectomycorrhizal Fungi and Their Effect on Nursery-Inoculated Pinus pinaster in a Post-fire Plantation in Northern Portugal

  • Environmental Microbiology
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Abstract

Ectomycorrhizal fungi (ECMF) play an important role in forest ecosystems, often mitigating stress factors and increasing seedling performance. The aim of this study was to investigate the effects of a nursery inoculation on Pinus pinaster growth and on the fungal communities established when reforesting burned areas. Inoculated P. pinaster saplings showed 1.5-fold higher stem height than the non-inoculated controls after a 5 year growth period, suggesting that fungal inoculation could potentiate tree growth in the field. Ordination analysis revealed the presence of different ECMF communities on both plots. Among the nursery-inoculated fungi, Laccaria sp., Rhizopogon sp., Suillus bovinus and Pisolithus sp. were detected on inoculated Pinus saplings on both sampling periods, indicating that they persisted after field establishment. Other fungi were also detected in the inoculated plants. Phialocephala sp. was found on the first assessment, while Terfezia sp. was detected on both sampling periods. Laccaria sp. and Rhizopogon sp. were identified in the control saplings, belonging however to different species than those found in the inoculated plot. Inocybe sp., Thelephora sp. and Paxillus involutus were present on both sampling periods in the non-inoculated plots. The results suggest that ECMF inoculation at nursery stage can benefit plant growth after transplantation to a post-fire site and that the inoculated fungi can persist in the field. This approach has great potential as a biotechnological tool to aid in the reforestation of burned areas.

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Acknowledgments

A.R. Franco, R. S. Oliveira and N.R. Sousa had the support of FCT grants SFRH/BD/47722/2008, SFRH/BPD/85008/2012, SFRH/BPD/89112/2012. This work was supported by National Funds through FCT—Fundação para a Ciência e a Tecnologia—under the project PTDC/AGR/CFL/111583/2009, project PEst-OE/EQB/LA0016/2013 and Fundo Social Europeu (III Quadro Comunitário de Apoio).

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  1. CBQF – Centro de Biotecnologia e Química Fina – Laboratório Associado, Escola Superior de Biotecnologia, Universidade Católica Portuguesa, Porto. Rua Dr. António Bernardino Almeida, 4200-072, Porto, Portugal

    Albina R. Franco, Nadine R. Sousa, Miguel A. Ramos, Rui S. Oliveira & Paula M. L. Castro

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Franco, A.R., Sousa, N.R., Ramos, M.A. et al. Diversity and Persistence of Ectomycorrhizal Fungi and Their Effect on Nursery-Inoculated Pinus pinaster in a Post-fire Plantation in Northern Portugal. Microb Ecol 68, 761–772 (2014). https://doi.org/10.1007/s00248-014-0447-9

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