Abstract
Waterways should be considered in the migration routes of Campylobacter, and the genus has been isolated from several water sources. Inferences on migration routes can be made from tracking genetic types in populations found in specific habitats and testing how they are linked to other types. Water samples were taken over a 4-year period from waterways in the Upper Oconee River Watershed, Georgia, to recover isolates of thermophilic Campylobacter. The isolates were typed by multilocus sequence typing (MLST) and analyzed to determine the overall diversity of Campylobacter in that environment. Forty-seven independent isolates were recovered from 560 samples (8.4 %). Two (~4 %) isolates were Campylobacter coli, three (~6 %) isolates were putatively identified as Campylobacter lari, and the remaining 42 (~90 %) were Campylobacter jejuni. The C. jejuni and C. coli isolates were typed by the Oxford MLST scheme. Thirty sequence types (STs) were identified including 13 STs that were not found before in the MLST database, including 24 novel alleles. Of the 17 previously described STs, 10 have been isolated from humans, 6 from environmental water, and 6 from wild birds (five types from multiple sources). Seven sites had multiple positive samples, and on two occasions, the same ST was isolated at the same site. The most common type was STST61 with four isolates, and the most common clonal complex was CC179 with nine isolates. CC179 has been commonly associated with environmental water. Although some Campylobacter STs that were found in the Oconee River engage in widespread migration, most are tightly associated with or unique to environmental water sources.
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References
Centers for Disease Control and Prevention (CDC) (2011) Vital signs: incidence and trends of infection with pathogens transmitted commonly through food—Foodborne Diseases Active Surveillance Network, 10 US sites, 1996–2010. MMWR Morb Mortal Wkly Rep 60:749–755
Schonberg-Norio D, Takkinen J, Hanninen ML, Katila ML, Kaukoranta SS, Mattila L, Rautelin H (2004) Swimming and Campylobacter infections. Emerg Infect Dis 10:1474–1477
Henderson PA, Seaby RMH (2002) Species diversity and richness III. Pisces Conservation Ltd. http://www.pisces-conservation.com/
Hepworth PJ, Ashelford KE, Hinds J, Gould KA, Witney AA, Williams NJ, Leatherbarrow H, French NP, Birtles RJ, Mendonca C, Dorrell N, Wren BW, Wigley P, Hall N, Winstanley C (2011) Genomic variations define divergence of water/wildlife-associated Campylobacter jejuni niche specialists from common clonal complexes Environ Microbiol 10–2920
Koenraad PMF, Rombouts FM, Notermans SHW (1997) Epidemiological aspects of thermophilic Campylobacter in water-related environments: a review. Water Environ Res 69:52–63
Levesque S, Frost E, Arbeit RD, Michaud S (2008) Multilocus sequence typing of Campylobacter jejuni isolates from humans, chickens, raw milk, environmental water in Quebec, Canada. J Clin Microbiol 46:3404–3411
Meinersmann RJ, Berrang ME, Jackson CR, Fedorka-Cray P, Ladely S, Little E, Frye JG, Mattsson B (2008) Salmonella, Campylobacter and Enterococcus spp.: their antimicrobial resistance profiles and their spatial relationships in a synoptic study of the Upper Oconee River Basin. Microb Ecol 55:444–452
Svensson SL, Gaynor EC FE (2008) Survival strategies of Campylobacter jejuni: Stress responses, the viable but nonculturable state, and biofilms. In: Nachamkin I, Szymanski CM, Blaser MJ (eds) Campylobacter, 3rd edn. ASM Press, Washington DC, pp 571–590
Miller WG, Englen MD, Kathariou S, Wesley IV, Wang G, Pittenger-Alley L, Siletz RM, Muraoka W, Fedorka-Cray PJ, Mandrell RE (2006) Identification of host-associated alleles by multilocus sequence typing of Campylobacter coli strains from food animals. Microbiology 152(Pt 1):245–255
Sheppard SK, McCarthy ND, Falush D, Maiden MC (2008) Convergence of Campylobacter species: implications for bacterial evolution. Science 320:237–239
Chan K, Elhanafi D, Kathariou S (2008) Genomic evidence for interspecies acquisition of chromosomal DNA from Campylobacter jejuni by Campylobacter coli strains of a turkey-associated clonal group (cluster II). Foodborne Pathog Dis 5(4):387–398
Koene MG, Houwers DJ, Dijkstra JR, Duim B, Wagenaar JA (2004) Simultaneous presence of multiple Campylobacter species in dogs. J Clin Microbiol 42:819–821
Richardson JF, Frost JA, Kramer JM, Thwaites RT, Bolton FJ, Wareing DR, Gordon JA (2001) Coinfection with Campylobacter species: an epidemiological problem? J Appl Microbiol 91:206–211
Dingle K, Colles FM, Wareing DR, Ure R, Fox AJ, Bolton FE, Bootsma HJ, Willems RJ, Urwin R, Maiden MC (2001) Multilocus sequence typing system for Campylobacter jejuni. J Clin Microbiol 39:14–23
Stern NJ, Wojton B, Kwiatek K (1992) A differential-selective medium and dry ice-generated atmosphere for recovery of Campylobacter jejuni. J Food Prot 55:514–517
Jolley KA, Maiden MC (2010) BIGSdb: scalable analysis of bacterial genome variation at the population level BMC Bioinformatics 11:595
Excoffier L, Laval G, Schneider S (2005) Arlequin ver 30: an integrated software package for population genetics data analysis. Evol Bioinformatics Online 1:47–50
Librado P, Rozas J (2009) DnaSP v5: a software for comprehensive analysis of DNA polymorphism data. Bioinformatics 25:1451–1452
Caro-Quintero A, Rodriguez-Castaño GP, Konstantinidis KT (2009) Genomic insights into the convergence and pathogenicity factors of Campylobacter jejuni and Campylobacter coli species. J Bacteriol 191:5824–5831
French N, Barrigas M, Brown P, Ribiero P, Williams N, Leatherbarrow H, Birtles R, Bolton E, Fearnhead P, Fox A (2005) Spatial epidemiology and natural population structure of Campylobacter jejuni colonizing a farmland ecosystem. Environ Microbiol 7:1116–1126
Meinersmann RJ, Dingle KE, Maiden MCJ (2003) Genetic exchange among Campylobacter species. Genome Lett 2:48–52
Acknowledgments
The authors are grateful to the volunteers of the Upper Oconee Watershed Network (UOWN), Rebecca Lindsey, Eric Adams, Caleb Lilly, Scott Ladely, Alice Wilcher, and Leena Jain for their assistance in collecting the samples. We are also thankful to Linda Genzlinger for her assistance in performing the DNA sequencing.
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Meinersmann, R.J., Berrang, M.E. & Little, E. Campylobacter spp. Recovered from the Upper Oconee River Watershed, Georgia in a 4-Year Study. Microb Ecol 65, 22–27 (2013). https://doi.org/10.1007/s00248-012-0117-8
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DOI: https://doi.org/10.1007/s00248-012-0117-8