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Unique Microbial Signatures of the Alien Hawaiian Marine Sponge Suberites zeteki

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Abstract

Invasive species poses a threat to the world’s oceans. Alien sponges account for the majority of introduced marine species in the isolated Hawaiian reef ecosystems. In this study, cultivation-dependent and cultivation-independent techniques were applied to investigate microbial consortia associated with the alien Hawaiian marine sponge Suberites zeteki. Its microbial communities were diverse with representatives of Actinobacteria, Firmicutes, α- and γ-Proteobacteria, Bacteroidetes, Chlamydiae, Planctomycetes, and Cyanobacteria. Specifically, the genus Chlamydia was identified for the first time from marine sponges, and two genera (Streptomyces and Rhodococcus) were added to the short list of culturable actinobacteria from sponges. Culturable microbial communities were dominated by Bacillus species (63%) and contained actinobacterial species closely affiliated with those from habitats other than marine sponges. Cyanobacterial clones were clustered with free-living cyanobacteria from water column and other environmental samples; they show no affiliation with other sponge-derived cyanobacteria. The low sequence similarity of Planctomycetes, Chlamydiae, and α-Proteobacteria clones to other previously described sequences suggested that S. zeteki may contain new lineages of these bacterial groups. The microbial diversity of S. zeteki was different from that of other studied marine sponges. This is the first report on microbial communities of alien marine invertebrate species. For the first time, it provides an insight into microbial structure within alien marine sponges in the Hawaiian marine ecosystems.

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Acknowledgments

We thank L. Zhang for assistance on phylogenetic analysis and D. Henderson for suggestions to greatly improve this manuscript. This work is funded by NOAA grant NA04OAR4600196 and University of Hawaii Sea Grant under institutional grants NA05OAR4171048 and NA16RG2254. The views expressed herein are those of the authors and do not necessarily reflect the views of NOAA or any of its subagencies.

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Authors and Affiliations

  1. Department of Biosynthesis of Natural Products, Institute of Materia Medica, Chinese Academy of Medical Sciences, Beijing, 100050, People’s Republic of China

    Ping Zhu

  2. Peking Union Medical College, Beijing, People’s Republic of China

    Ping Zhu

  3. Department of Oceanography, University of Hawaii at Manoa, Honolulu, HI, 96822, USA

    Ping Zhu, Quanzi Li & Guangyi Wang

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  1. Ping Zhu
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  2. Quanzi Li
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Correspondence to Guangyi Wang.

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Zhu, P., Li, Q. & Wang, G. Unique Microbial Signatures of the Alien Hawaiian Marine Sponge Suberites zeteki . Microb Ecol 55, 406–414 (2008). https://doi.org/10.1007/s00248-007-9285-3

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