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Archaeal Communities in a Tropical Estuarine Ecosystem: Guanabara Bay, Brazil

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Abstract

Guanabara Bay is an eutrophic estuarine system located in a humid tropical region surrounded by the second largest metropolitan area of Brazil. This study explores the contrasting environmental chemistry and microbiological parameters that influence the archaeaplankton diversity in a pollution gradient in Guanabara Bay ecosystem. The environments sampled ranged from completely anoxic waters in a polluted inner channel to the adjacent, relatively pristine, coastal Atlantic Ocean. Partial archaeal 16S rDNA sequences in water samples were retrieved by polymerase chain reaction (PCR) and analyzed using denaturing gradient gel electrophoresis (DGGE), cloning, and sequencing. Sequences were subjected to phylogenetic and diversity analyses. Community structure of the free-living archaeal assemblages was different from that of the particle-attached archaea according to DGGE. Gene libraries revealed that phylotype identification was consistent with environmental setting. Archaeal phylotypes found in polluted anoxic waters and in more pristine waters were closely related to organisms that have previously been found in these environments. However, inner bay archaea were related to organisms found in oil, industrial wastes, and sewage, implying that water pollution controls archaea communities in this system. The detection of a substantial number of uncultured phylotypes suggests that Guanabara Bay harbors a pool of novel archaeaplankton taxa.

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Acknowledgments

We acknowledge Genome Sequencing facilities core Johanna Döbereiner, IBqM/UFRJ, Limnology Laboratory of UFRJ for the access to liquid scintillator and officers and crew of OV Astro Garoupa. We are grateful to PETROBRAS and Astromarítima for kindly providing ship time and fuel. We also thank Leonardo Cesar A. da Silva for his valuable help with Rarefaction analysis program. We are grateful to Fernando N. Pinto for sample collection and Guilherme R. S. Muricy for manuscript review. We also thank Laboratory of Ecology and Taxonomy of Microorganisms IMPPG/UFRJ for DGGE facilities. This work was supported by a FAPERJ grant (150.121/04); RHAE—PUC; Pronex 171.177/03 (CNPq/FAPERJ); Finep/CTPetro No. 21.01.0278.00.

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Authors and Affiliations

  1. Instituto de Biologia, Departamento de Biologia Marinha, Universidade Federal do Rio de Janeiro, Rio de Janeiro, 21941-590, Brazil

    Ricardo P. Vieira, Alessandra M. Gonzalez & Rodolfo Paranhos

  2. Instituto de Bioquímica Médica, Universidade Federal do Rio de Janeiro, Rio de Janeiro, 21941-590, Brazil

    Ricardo P. Vieira, Alexander M. Cardoso & Orlando B. Martins

  3. Instituto Nacional de Controle da Qualidade em Saúde—INCQS/FIOCRUZ, Rio de Janeiro, 21045-900, Brazil

    Maysa M. Clementino

  4. Departamento de Bioquímica, Universidade do Estado do Rio de Janeiro, Rio de Janeiro, 20551-013, Brazil

    Denise N. Oliveira & Rodolpho M. Albano

Authors
  1. Ricardo P. Vieira
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  2. Maysa M. Clementino
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  3. Alexander M. Cardoso
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  4. Denise N. Oliveira
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  6. Alessandra M. Gonzalez
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  7. Rodolfo Paranhos
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  8. Orlando B. Martins
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Corresponding author

Correspondence to Alexander M. Cardoso.

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Vieira, R.P., Clementino, M.M., Cardoso, A.M. et al. Archaeal Communities in a Tropical Estuarine Ecosystem: Guanabara Bay, Brazil. Microb Ecol 54, 460–468 (2007). https://doi.org/10.1007/s00248-007-9261-y

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  • DOI: https://doi.org/10.1007/s00248-007-9261-y

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