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An Endolithic Microbial Community in Dolomite Rock in Central Switzerland: Characterization by Reflection Spectroscopy, Pigment Analyses, Scanning Electron Microscopy, and Laser Scanning Microscopy

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Abstract

A community of endolithic microorganisms dominated by phototrophs was found as a distinct band a few millimeters below the surface of bare exposed dolomite rocks in the Piora Valley in the Alps. Using in situ reflectance spectroscopy, we detected chlorophyll a (Chl a), phycobilins, carotenoids, and an unknown type of bacteriochlorophyll-like pigment absorbing in vivo at about 720 nm. In cross sections, the data indicated a defined distribution of different groups of organisms perpendicular to the rock surface. High-performance liquid chromatography analyses of pigments extracted with organic solvents confirmed the presence of two types of bacteriochlorophylls besides chlorophylls and various carotenoids. Spherical organisms of varying sizes and small filaments were observed in situ with scanning electron microscopy and confocal laser scanning microscopy (one- and two-photon technique). The latter allowed visualization of the distribution of phototrophic microorganisms by the autofluorescence of their pigments within the rock. Coccoid cyanobacteria of various sizes predominated over filamentous ones. Application of fluorescence-labeled lectins demonstrated that most cyanobacteria were embedded in an exopolymeric matrix. Nucleic acid stains revealed a wide distribution of small heterotrophs. Some biological structures emitting a green autofluorescence remain to be identified.

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Acknowledgments

We thank Drs. F. Schanz and M. Wiggli for fruitful discussions, the help concerning statistical calculations, and treatment of the in vivo spectra. We are much indebted to Dr. B. Wise for his help in clarifying the English text and Dr. J. Blom for the HPLC analyses. The excellent technical assistance of U. Kuhlicke (LSM) and U. Jauch (SEM) is appreciated.

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Authors and Affiliations

  1. Institute of Plant Biology/Microbiology, University of Zürich, Zollikerstrasse 107, CH-8008, Zürich, Switzerland

    T. Horath & R. Bachofen

  2. Department of River Ecology, UFZ Center for Environmental Research, Leipzig-Halle, Brückstrasse 3A, D-39114, Magdeburg, Germany

    T. R. Neu

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  1. T. Horath
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Correspondence to R. Bachofen.

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Horath, T., Neu, T.R. & Bachofen, R. An Endolithic Microbial Community in Dolomite Rock in Central Switzerland: Characterization by Reflection Spectroscopy, Pigment Analyses, Scanning Electron Microscopy, and Laser Scanning Microscopy. Microb Ecol 51, 353–364 (2006). https://doi.org/10.1007/s00248-006-9051-y

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  • DOI: https://doi.org/10.1007/s00248-006-9051-y

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