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Genetic and Physiological Characterization of the Intestinal Bacterial Microbiota of Bluegill (Lepomis macrochirus) with Three Different Feeding Habits

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Abstract

Bluegill (Lepomis macrochirus) in Lake Biwa, Japan, feed on benthic invertebrates (benthivorous type), aquatic plants (herbivorous type), and zooplankton (planktivorous type). To evaluate the effect of food on intestinal bacterial microbiota, we characterized and compared the intestinal microbiota of these three types of bluegill in terms of community-level physiological profile (CLPP) and genetic structure. The CLPP was analyzed using Biolog MicroPlates (Biolog, Inc., Hayward, CA, USA), and multivariate analysis of variance revealed that the CLPP of intestinal microbiota differed significantly between any pairs of the three types of bluegill. The genetic profiles were analyzed by temperature gradient gel electrophoresis of polymerase chain reaction (PCR)-amplified 16S rDNA fragments, and multidimensional scaling indicated the existence of specific intestinal bacterial structures for both the benthivorous and the planktivorous types. These results suggest that the host's feeding habit can be one factor controlling the intestinal microbiota of fish in the natural environment.

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Acknowledgments

We greatly acknowledge S. Gritters and K. Aulwes, Iowa Department of Natural Resources, for fish sampling in Iowa. We thank N. Yamaguchi, C. Sakamoto, F. Maruyama, and other members of Osaka University for their help in the TGGE experiments. We also thank M. Yuma for reviewing an earlier draft of this manuscript, and M. Honjo and M. Ueki for helpful comments and technical assistance. This research was partly supported by the Grant for the Biodiversity Research of the 21st Century COE (A14). The study was also partly supported by the JSPS Grant-in Aid for Basic Research (A) (16207001).

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Author notes

  1. Zen'ichiro Kawabata

    Present address: Research Institute for Humanity and Nature, Kamigyo-ku, Kyoto, 602-0878, Japan

Authors and Affiliations

  1. Center for Ecological Research, Kyoto University, 509-3, 2-chome, Hirano, Otsu, Shiga, 520-2113, Japan

    Kimiko Uchii & Zen'ichiro Kawabata

  2. Laboratory of Environmental Biotechnology, Faculty of Engineering, Tohoku Gakuin University, Tagajo, Miyagi, 985-8537, Japan

    Kazuaki Matsui

  3. Gifu Prefectural Research Institute for Freshwater Fish and Aquatic Environments, Kakamigahara, Gifu, 501-6021, Japan

    Ryuji Yonekura

  4. Environmental Science and Microbiology, Graduate School of Pharmaceutical Sciences, Osaka University, Suita, Osaka, 565-0871, Japan

    Katsuji Tani, Takehiko Kenzaka & Masao Nasu

Authors
  1. Kimiko Uchii
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  2. Kazuaki Matsui
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  3. Ryuji Yonekura
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  4. Katsuji Tani
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  5. Takehiko Kenzaka
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  6. Masao Nasu
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  7. Zen'ichiro Kawabata
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Correspondence to Kimiko Uchii.

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Uchii, K., Matsui, K., Yonekura, R. et al. Genetic and Physiological Characterization of the Intestinal Bacterial Microbiota of Bluegill (Lepomis macrochirus) with Three Different Feeding Habits. Microb Ecol 51, 277–284 (2006). https://doi.org/10.1007/s00248-006-9018-z

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  • DOI: https://doi.org/10.1007/s00248-006-9018-z

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