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Abundance of Three Bacterial Populations in Selected Streams

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Abstract

The population sizes of three bacterial species, Acinetobacter calcoaceticus, Burkholderia cepacia, and Pseudomonas putida, were examined in water and sediment from nine streams in different parts of the United States using fluorescent in situ hybridization (FISH). Population sizes were determined from three sites (upstream, midstream, and downstream) in each stream to compare differences in the occurrence and distribution of the species within each stream and among streams. Physical and chemical variables measured reflected differences in environmental conditions among the streams. In the water, B. cepacia numbers were highest in the agricultural, Iowa stream. P. putida numbers were highest in the southern coastal plain streams, Black Creek (GA) and Meyers Branch (SC). Compared to the other two species, the abundance of A. calcoaceticus was similar in all the streams. In sediment, the greatest abundance of all three species was found in the Iowa stream, while the lowest was in Hugh White Creek (NC). Detrended correspondence analysis (DCA) explained 95.8% and 83.9% of the total variation in bacterial numbers in water and sediment of the streams, respectively. In sediments and water, B. cepacia numbers were related to nitrate concentrations. A. calcoaceticus in water clustered with several environmental variables (i.e., SRP, pH, and conductivity) but benthic populations were less well correlated with these variables. This study reveals the potential influence of various environmental conditions on different bacterial populations in stream communities.

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Acknowledgments

This research was supported by a National Science Foundation grant (DEB-0087051). We thank Adam Leff, Mark Crissman, Mandy Brothers, Angela Lindell, Brian Kloeppel, Leonard Smock, Anne Wright, Tim Kratz, Stephen Hamilton, and Walter Dodds for field assistance. We thank the Savannah River Ecology Laboratory, Coweeta Hydrological Laboratory, Department of Biology at Virginia Commonwealth University, Trout Lake Station at University of Wisconsin, Kellogg Biological Station at Michigan State University, and Konza Prairie Biological Station at Kansas State University for providing access to study sites.

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  1. X. Gao

    Present address: Florida Department of Environmental Protection, 2600 Blair Stone Road, Tallahassee, FL, 32399, USA

Authors and Affiliations

  1. Department of Biological Sciences, Kent State University, Kent, OH, 44242, USA

    O.A. Olapade, X. Gao & L.G. Leff

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  1. O.A. Olapade
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  2. X. Gao
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  3. L.G. Leff
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Correspondence to O.A. Olapade.

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Olapade, O., Gao, X. & Leff, L. Abundance of Three Bacterial Populations in Selected Streams. Microb Ecol 49, 461–467 (2005). https://doi.org/10.1007/s00248-004-0030-x

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