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Genetic Diversity in Microcystis Populations of a French Storage Reservoir Assessed by Sequencing of the 16S-23S rRNA Intergenic Spacer

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Abstract

We compared the genetic diversity of the 16S-23S spacer of the rRNA gene (ITS1) in benthic and pelagic colonies of the Microcystis genus isolated from two different sampling stations with different depths and at two different sampling times (winter and summer) in the French storage reservoir of Grangent. In all, 66 ITS1 sequences were found in the different clone libraries. The nucleotide diversity of all the sampled isolates were in the same range (average number = 0.022) regardless of their origin, showing that several clones are involved in the summer bloom event and contribute to the high biomass production. Phylogenetic study and analysis of molecular variance (AMOVA) revealed no obvious genetic differentiation between the benthic and pelagic isolates. This finding confirms that the Microcystis genus in this lake is characterized by having both a benthic phase in winter and spring allowing this organism to survive in unfavorable environmental conditions, and a pelagic phase in summer and autumn when environmental conditions allow them to grow in the water column. Finally, comparing these sequences with those available in the GenBank database showed that some highly conserved genotypes are found throughout the world.

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Acknowledgments

We thank S. Gay for technical assistance and M. Gugger for stimulating discussions of the manuscript. The English text has been checked by Monika Ghosh.

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Authors and Affiliations

  1. INRA, UMR CARRTEL, BP 511, 74203, Thonon Cedex, France

    J.F. Humbert & B. Le Berre

  2. Université J. Monnet, Lab. de Biologie Animale et Appliquée, 42023, St Etienne, France

    D. Duris-Latour & H. Giraudet

  3. EDFR&D, Laboratoire National d’Hydraulique et Environnement, 6, Quai Watier, 78401, Chatou Cedex, France

    M.J. Salençon

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  1. J.F. Humbert
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Correspondence to J.F. Humbert.

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Humbert, J., Duris-Latour, D., Berre, B.L. et al. Genetic Diversity in Microcystis Populations of a French Storage Reservoir Assessed by Sequencing of the 16S-23S rRNA Intergenic Spacer. Microb Ecol 49, 308–314 (2005). https://doi.org/10.1007/s00248-004-0004-z

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