Abstract
Pseudomonas aeruginosa is a pathogenic bacterium that has been thoroughly investigated since the 19th century and is generally regarded as a freshwater or terrestrial organism. In 1995, it was reported that the OprP porin, an outer membrane protein corresponding to that of this bacterium, was widely distributed as a dissolved component in seawater. This finding led us to investigate the presence of P. aeruginosa in marine environments. Both culture-independent and -dependent methods were applied to seawater samples obtained in Tokyo Bay during four cruises. The DVC-FA (direct viable count–fluorescent antibody) technique showed that cells reactive to an antibody against P. aeruginosa were widely present in the bay, i.e., 103 to 104 cells/mL in the inner bay, and 102 to 103 cells/mL at the mouth. Bacterial cells isolated by selective medium were identified by three methods: the presence of oprI and oprL, two outer membrane lipoprotein genes specific to P. aeruginosa; the API20 NE kit; and 16S rDNA sequence analysis. The results confirmed that the majority of isolates from the bay were P. aeruginosa. Immuno-chemical analyses of the seawater results indicate that P. aeruginosa is commonly present in coastal marine environments and sheds OprP.
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Acknowledgements
P. aeruginosa PAO1 was a kind gift from the Pseudomonas Genome Project. This work was partly supported by Grant-in-Aid for Creative Basic Research No. 12NP0201 “Dynamics of the Ocean Biosystem (DOBIS)” and by Grant-in-Aid for Basic Research no. 12490009 from Ministry of Education, Culture, Sports, Science, and Technology (MEXT), Japan.
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Kimata, N., Nishino, T., Suzuki, S. et al. Pseudomonas aeruginosa Isolated from Marine Environments in Tokyo Bay . Microb Ecol 47, 41–47 (2004). https://doi.org/10.1007/s00248-003-1032-9
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DOI: https://doi.org/10.1007/s00248-003-1032-9