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Characterization of Humus Microbial Communities in Adjacent Forest Types That Differ in Nitrogen Availability

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Abstract

To address the link between soil microbial community composition and soil processes, we investigated the microbial communities in forest floors of two forest types that differ substantially in nitrogen availability. Cedar-hemlock (CH) and hemlock-amabilis fir (HA) forests are both common on northern Vancouver Island, B.C., occurring adjacently across the landscape. CH forest floors have low nitrogen availability and HA high nitrogen availability. Total microbial biomass was assessed using chloroform fumigation-extraction and community composition was assessed using several cultivation-independent approaches: denaturing gradient gel electrophoresis (DGGE) of the bacterial communities, ribosomal intergenic spacer analysis (RISA) of the bacterial and fungal communities, and phospholipid fatty acid (PLFA) profiles of the whole microbial community. We did not detect differences in the bacterial communities of each forest type using DGGE and RISA, but differences in the fungal communities were detected using RISA. PLFA analysis detected subtle differences in overall composition of the microbial community between the forest types, as well as in particular groups of organisms. Fungal PLFAs were more abundant in the nitrogen-poor CH forests. Bacteria were proportionally more abundant in HA forests than CH in the lower humus layer, and Gram-positive bacteria were proportionally more abundant in HA forests irrespective of layer. Bacterial and fungal communities were distinct in the F, upper humus, and lower humus layers of the forest floor and total biomass decreased in deeper layers. These results indicate that there are distinct patterns in forest floor microbial community composition at the landscape scale, which may be important for understanding nutrient availability to forest vegetation.

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Acknowledgements

We thank C. Staley and S. Adair for field assistance, D. Adams (Nucleic Acid and Protein Services Unit, UBC), and B. Ord (Macaulay Land Use Research Institute, UK) for assisting with the RISA and PLFA analyses, respectively, and S. Harper and P. Parkinson (Environmental Engineering Lab, UBC) for the soluble organic carbon analyses. Western Forest Products, Ltd., provided field assistance and accommodations. The project was funded by Forest Renewal British Columbia and Forestry Innovation Investment grants to C.E.P., Scottish Executive Environment and Rural Affairs Department grants to S.J.G., and Natural Sciences and Engineering Research Council and Edward W. Bassett Memorial scholarships to S.E.L.

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  1. S. J. Grayston

    Present address: Department of Forest Sciences, University of British Columbia, 2424 Main Mall, Vancouver, BC, V6T 1Z4, Canada

Authors and Affiliations

  1. Department of Forest Sciences, University of British Columbia, 2424 Main Mall, Vancouver, BC, V6T 1Z4, Canada

    S. E. Leckie & C. E. Prescott

  2. Macaulay Land Use Research Institute, Aberdeen, AB15 8QH, UK

    S. J. Grayston

  3. Department of Microbiology and Immunology, University of British Columbia, #300-6174 University Boulevard, Vancouver, BC, V6T 1Z3, Canada

    J. D. Neufeld & W. W. Mohn

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  1. S. E. Leckie
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Leckie, S., Prescott, C., Grayston, S. et al. Characterization of Humus Microbial Communities in Adjacent Forest Types That Differ in Nitrogen Availability . Microb Ecol 48, 29–40 (2004). https://doi.org/10.1007/s00248-003-1020-0

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