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Identification of Planctomycetes with Order-, Genus-, and Strain-Specific 16S rRNA-Targeted Probes

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Abstract

A specific 16S rRNA-targeted oligonucleotide probe (PIR1223) for the genus Pirellula and a species-specific probe (RB454) for Pirellula sp. strain SH1 have been designed and optimized. Together with the already existing order-specific probe PLA886, the two newly designed probes were used to detect and identify planctomycetes, pirellulae, and close relatives of Pirellula sp. strain SH1 in different habitats. With the help of these probes for detection and identification, bacteria of the genus Pirellula were detected and cultivated from tissue of the Mediterranean sponge Aplysina aerophoba and from the water column of the Kiel Fjord. An unexpected result was the close phylogenetic relationship of the isolate from the sponge and the brackish water habitat Kiel Fjord as revealed by DNA/DNA hybridization.

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Acknowledgements

We gratefully acknowledge U. Hentschel and A.B. Friedrich from the Institut für Molekulare Infektionsbiologie, Universität Würzburg, for providing the sponge material and for the preparation and fixation of the sponge material for FISH studies.

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Authors and Affiliations

  1. Max-Planck-Institut für Marine Mikrobiologie, Celsiusstr. 1, D-28359 Bremen, Germany

    D. Gade, F. O. Glöckner & R. Amann

  2. Institut für Allgemeine Mikrobiologie, Universität Kiel, Am Botanischen Garten 1-9, D-24118 Kiel, Germany

    D. Gade, H. Schlesner & M. Thomm

  3. Zentrale Mikroskopie, Biologiezentrum der Universität Kiel, Am Botanischen Garten 5, D-24098 Kiel, Germany

    S. Pfeiffer

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  1. D. Gade
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Correspondence to D. Gade.

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Gade, D., Schlesner, H., Glöckner, F. et al. Identification of Planctomycetes with Order-, Genus-, and Strain-Specific 16S rRNA-Targeted Probes . Microb Ecol 47, 243–251 (2004). https://doi.org/10.1007/s00248-003-1016-9

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