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Correlative BOLD MR imaging of stages of synovitis in a rabbit model of antigen-induced arthritis

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Abstract

Background

Because of the ability of blood-oxygen-level-dependent (BOLD) MRI to assess blood oxygenation changes within the microvasculature, this technique holds potential for evaluating early perisynovial changes in inflammatory arthritis.

Objective

To evaluate the feasibility of BOLD MRI to detect interval perisynovial changes in knees of rabbits with inflammatory arthritis.

Materials and methods

Rabbit knees were injected with albumin (n = 9) or saline (n = 6) intra-articularly, or were not injected (control knees, n = 9). Except for two rabbits (albumin-injected, n = 2 knees; saline-injected, n = 2 knees) that unexpectedly died on days 7 and 21 of the experiment, respectively, all other animals were scanned with BOLD MRI on days 0, 1, 7, 14, 21 and 28 after induction of arthritis. T2*-weighted gradient-echo MRI was performed during alternate 30 s of normoxia/hyperoxia. BOLD MRI measurements were compared with clinical, laboratory and histological markers.

Results

Percentage of activated voxels was significantly greater in albumin-injected knees than in contralateral saline-injected knees (P = 0.04). For albumin-injected knees (P < 0.05) and among different categories of knees (P = 0.009), the percentage of activated BOLD voxels varied over time. A quadratic curve for on-and-off BOLD difference was delineated for albumin- and saline-injected knees over time (albumin-injected, P = 0.047; saline-injected, P = 0.009). A trend toward a significant difference in synovial histological scores between albumin-injected and saline-injected knees was noted only for acute scores (P = 0.07).

Conclusion

As a proof of concept, BOLD MRI can depict perisynovial changes during progression of experimental arthritis.

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Acknowledgements

The authors thank Michael Noseworthy for setting up the MR sequences used in this study and for reviewing the first draft of the manuscript, Sharon Nancekivell for editorial assistance, Dr. Brian Feldman for advice about the design of the study, Dr. Geraldine Kent for help with the preparation of the albumin solution, Marianne Rogers for histological services, Jeff Alfonsi and Niels Celeghin for analysis of data, Man Khun Chan and Dr. Adele Khoskow for suggesting and testing laboratory surrogates for inflammation in rabbits, and Hillary Bruce and Mary Ancona for collaborating as MR imaging technologists in part of the study.

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Authors and Affiliations

  1. Department of Diagnostic Imaging, The Hospital for Sick Children, 555 University Ave., Toronto, Canada, M5G1X8

    Andrea S. Doria, Harpal Gahunia, Tammy Rayner, Vivian Tassos & Anguo Zhong

  2. Department of Orthopedic Surgery, The Hospital for Sick Children, Toronto, Canada

    Robert B. Salter

  3. Department of Medical Imaging, University of Toronto, Toronto, Canada

    Andrea S. Doria & Adrian Crawley

  4. Department of Medical Imaging, Toronto Western Hospital, Toronto, Canada

    Adrian Crawley

  5. Department of Public Health, Family and Community Medicine, Toronto, Canada

    Rahim Moineddin

  6. Department of Pathology and Laboratory Medicine, Mount Sinai Hospital, Toronto, Canada

    Kenneth Pritzker, Maria Mendes & Roland Jong

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  1. Andrea S. Doria
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Correspondence to Andrea S. Doria.

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In memoriam of Robert B. Salter.

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Doria, A.S., Crawley, A., Gahunia, H. et al. Correlative BOLD MR imaging of stages of synovitis in a rabbit model of antigen-induced arthritis. Pediatr Radiol 42, 63–75 (2012). https://doi.org/10.1007/s00247-011-2194-0

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  • DOI: https://doi.org/10.1007/s00247-011-2194-0

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