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Genotoxic Evaluation in Tadpoles Associated with Agriculture in the Central Cerrado, Brazil

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Abstract

Many agricultural practices cause environmental degradation that affects the cellular integrity of anurans. In the present study, we provided in situ data of Dendropsophus minutus, Physalaemus cuvieri, and Scinax fuscovarius collected in soybean/corn and conservation units in the Brazilian Cerrado. The in situ data showed no significant variation in the micronucleus frequency between the sites, only the reniform cells had a higher rate for the agricultural environment. A combined analysis of all nuclear erythrocyte abnormalities (ENAs = nuclear buds, reniform nuclei, apoptotic cell, binucleated, and anucleated cells) was recorded higher frequencies in farmland. Overall, Scinax fuscovarius was considered the best potential bioindicator for soybean/corn plantations. Finally, we recommend expanding the micronucleus test for in situ studies to expand our understanding of the sensitivity of native anuran species and provide a more systematic assessment of the adverse effects of environmental pollutants on wildlife.

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Acknowledgements

The authors are grateful to the Brazilian National Council for Scientific and Technological Development (CNPq), for supporting this study through personal research Grant Number 477044/2013-1, and the São Paulo State Research Foundation (FAPESP) for supporting the analyses (Process No. 2013/02067-5). They also thank the Chico Mendes Institute for Biodiversity Conservation (ICMBio/SISBIO) and the Emas National Park for authorizing fieldwork.

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Authors and Affiliations

  1. Department of Biology, Universidade de Rio Verde, UniRV, Fazenda Fontes do Saber, Rio Verde, GO, CEP 75.901-970, Brazil

    Rinneu Elias Borges & Richelle Sousa Modesto

  2. Laboratory of Animal Biology, Instituto Federal Goiano, IF Goiano, Campus Rio Verde, Rodovia Sul Goiana, Km 01, Zona Rural, Rio Verde, GO, CEP 75.901-970, Brazil

    Lia Raquel de Souza Santos, Marcelino Benvindo-Souza & Rhayane Alves Assis

  3. Department of Biology, Universidade Estadual Paulista - Júlio de Mesquita Filho, UNESP, Campus São José do Rio Preto, R. Cristóvão Colombo, 2265, Jardim Nazareth, São José do Rio Preto, SP, CEP 15.054-000, Brazil

    Classius de Oliveira

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  1. Rinneu Elias Borges
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  2. Lia Raquel de Souza Santos
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  3. Marcelino Benvindo-Souza
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  4. Richelle Sousa Modesto
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Correspondence to Rinneu Elias Borges.

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Borges, R.E., Santos, L.R.d., Benvindo-Souza, M. et al. Genotoxic Evaluation in Tadpoles Associated with Agriculture in the Central Cerrado, Brazil. Arch Environ Contam Toxicol 77, 22–28 (2019). https://doi.org/10.1007/s00244-019-00623-y

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