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Toxic Mechanisms Induced by Fumonisin B1 Mycotoxin on Human Intestinal Cell Line

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Abstract

The gastrointestinal tract is the main target of exposure to mycotoxin fumonisin B1 (FB1), common natural contaminant in food. Previous studies reported that proliferating cells are more sensitive than confluent cells to the toxic effect of FB1. This study aims to investigate, by dose- and time-dependent experiments on human colon proliferating intestinal cell line (HT-29), the modifications induced by FB1 at concentrations ranging from 0.25 to 69 μM. The choice of highest FB1 concentration considered the low toxicity previously reported on intestinal cell lines, whereas the lowest one corresponded to the lower FBs levels permitted by European Commission Regulation. Different functional parameters were tested such as cell proliferation, oxidative status, immunomodulatory effect and changes in membrane microviscosity. In addition FB1-FITC localization in this cell line was assessed by using confocal laser scanning microscopy. Lipid peroxidation induction was the main and early (12 h) effect induced by FB1 at concentrations ranging from 0.5 to 69 μM, followed by inhibition of cell proliferation (up to 8.6 μM), the immunomodulatory effect (up to 17.2 μM), by assessing IL-8 secretion, and increase in membrane microviscosity (up to 34.5 μM). The toxic effects observed in different functional parameters were not dose-dependent and could be the consequence of the FB1 intracytoplasmatic localization as confirmed by confocal microscopy results. The different timescales and concentrations active of different functional parameters could suggest different cellular targets of FB1.

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Acknowledgments

This work was supported in part by EC KBBE-2007-222690-2 Novel Integrated Strategies for worldwide mycotoxin reduction in food and feed chains (MycoRed).

Conflict of interest

The authors declare that there are no conflicts of interest.

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Authors and Affiliations

  1. Institute of Sciences of Food Production, National Research Council of Italy (CNR), Via Amendola 122/O, 70125, Bari, Italy

    Fiorenza Minervini, Antonella Garbetta, Isabella D’Antuono, Angela Cardinali & Angelo Visconti

  2. Department of Emergency and Organ Transplantation, Veterinary Clinics and Animal Production, University of Bari, Strada Provinciale per Casamassima, km 3, 70010, Valenzano, Bari, Italy

    Nicola Antonio Martino

  3. Department of Biosciences, Biotechnologies and Biopharmacology, University of Bari, Via Orabona 4, 70125, Bari, Italy

    Lucantonio Debellis

Authors
  1. Fiorenza Minervini
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  2. Antonella Garbetta
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  3. Isabella D’Antuono
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  4. Angela Cardinali
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  7. Angelo Visconti
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Correspondence to Fiorenza Minervini.

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Minervini, F., Garbetta, A., D’Antuono, I. et al. Toxic Mechanisms Induced by Fumonisin B1 Mycotoxin on Human Intestinal Cell Line. Arch Environ Contam Toxicol 67, 115–123 (2014). https://doi.org/10.1007/s00244-014-0004-z

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  • DOI: https://doi.org/10.1007/s00244-014-0004-z

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