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Genotoxicity in Astyanax bimaculatus (Twospot Astyanax) Exposed to the Waters of Engano River (Brazil) as Determined by Micronucleus Tests in Erythrocytes

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Abstract

Changes in aquatic environments are potentially large, and effects on biota are highly variable. In Santa Catarina State, Brazil, the main sources of pollution in headwaters are wastewater, pesticides, and animal waste, which contain chemical and organic additives, and there is little knowledge about their effects on aquatic organisms. This study was aimed at investigating the possible genotoxic effects of the waters of the Engano River through the micronucleus (MN) test in Astyanax bimaculatus erythrocytes. Samplings were performed at two sites on the river, and there were six temporal repetitions with collection of blood from six individuals per site/repetition. For the negative-control treatment, we used fish from organic culture; cyclophosphamide was inoculated to constitute the positive-control treatment. MN was obtained in 3,000 erythrocytes/individual. Kruskal–Wallis analysis of variance, Scott–Knott test, and Spearman correlation were employed for data analysis. We found 124 MN in fish from the river, with 70 and 54 MN, respectively, for each site and 16 and 59 MN in negative and positive controls, respectively. The results showed a statistical difference for the formation of MN between river fish and fish in the positive-control treatment. Considering the average MN, we found the formation of three distinct groups: (1) fish from site no. 1 and those in the negative-control group, (2) fish from site no. 2, and (3) fish in the positive-control group. The greater values of chromosomal damage were found during periods of lower rainfall. The test used indicated the presence in the water of substances likely to cause clastogenic and aneugenic effects.

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References

  • Al-Sabti K (1991) Handbook of genotoxic effects and fish chromosomes. Jozef Stefan Institute, Jamova

    Google Scholar 

  • Al-Sabti K, Metcalfe CD (1995) Fish micronuclei for assessing genotoxicity in water. Mutat Res 343:121–135

    Article  CAS  Google Scholar 

  • Anbumani S, Mohankumar MN (2012) Gamma radiation induced micronuclei and erythrocyte cellular abnormalities in the fish Catla catla. Aquat Toxicol 122:125–132

    Article  Google Scholar 

  • Anderson MJ, Ellingsen KE, McArdle BH (2006) Multivariate dispersion as a measure of beta diversity. Ecol Lett 9:683–693

    Article  Google Scholar 

  • Bartram J, Balance R (1996) Water quality monitoring: a practical guide to the design and implementation of freshwater quality studies and monitoring programmes. E&FN Spon, London, UK

  • Belpaeme K, Delbeke K, Zhu L, Kirsch-Volders M (1996) Cytogenetic studies of PCB 77 on brown trout (Salmo trutta fario) using the micronucleus test and the alkaline comet assay. Mutagenesis 11:485–492

    Article  CAS  Google Scholar 

  • Beyersmann D, Hartwig A (2008) Carcionogenic metal compounds: recent insight into molecular and cellular mechanisms. Arch Toxicol 82:493–512

    Article  CAS  Google Scholar 

  • Bucker A, Carvalho W, Alves-Gomes JA (2006) Avaliação da mutagênese e genotoxicidade em Eigenmannia virescens (Teleostei: Gymnotiformes) expostos ao benzeno. Acta Amazonica 36(3):357–364

    Article  CAS  Google Scholar 

  • Cardinale BJ, Duffy JE, Gonzalez A, Hooper DU, Perring C, Venail P et al (2012) Biodiversity loss and its impact on humanity. Nature 486:59–67

    Article  CAS  Google Scholar 

  • Chapin FS, Zavaletta ES, Eviner VT, Naylor RL, Vitousek PM, Reynolds HL et al (2000) Consequences of changing biodiversity. Nature 405:234–242

    Article  CAS  Google Scholar 

  • Da Rosa GA, Stolberg J, Piccinin IP (eds) (2009) Plano estratégico de gestão integrada da bacia hidrográfica do Rio Jacutinga—Etapa C. Governo do Estado de Santa Catarina, Secretaria de Estado de Desenvolvimento Sustentável, Diretoria de Recursos Hídricos, Florianópolis

  • De La Torre AI, Jiménez JA, Carballo M, Fernandez C, Roset J, Muñoz MJ (2000) Ecotoxicological evaluation of pig slurry. Chemosphere 41:1629–1635

    Article  Google Scholar 

  • Duarte ID, Dias MC, David JAO, Matsumoto ST (2012) A qualidade da água da Lagoa Jacuném (Espírito Santo, Brasil) em relação a aspectos genotóxicos e mutagênicos, mensurados respectivamente pelo ensaio do cometa e teste do micronúcleo em peixes da espécie Oreochromis niloticus. R Bras Bioci 10(2):211–219

    Google Scholar 

  • Fenech M (2000) The in vitro micronucleus technique. Mutat Res 455:81–95

    Article  CAS  Google Scholar 

  • Ferraro MVM, Fenochio AS, Mantovani MS, Ribeiro CO, Cestari MM (2004) Mutagenic effects of tributyltin and inorganic lead (Pb II) on the fish H. malabaricus evaluated using the comet assay and the piscine micronucleus and chromosome aberration tests. Genet Mol Biol 27(1):103–107

    Article  CAS  Google Scholar 

  • Ferreira GR, Nepomuceno JC (2008) Poluição do Rio Santa Catarina no município de Vazante (MG) detectada por meio do teste do micronúcleo em peixes. Perquirère 5(1):155–160

    Google Scholar 

  • Galindo BA, Trilo G, Cólus IMS, Martinez CBR, Sofia SH (2010) Genotoxic effects of aluminum on the neotropical fish Prochilodus lineatus. Water Air Soil Pollut 212(4):419–428

    Article  CAS  Google Scholar 

  • Grisolia CK (2005) Agrotóxicos: mutações, câncer & reprodução. Universidade de Brasília, Brasília

    Google Scholar 

  • Grisolia CK, Starling FLRM (2001) Micronuclei monitoring of fishes from Lake Paranoá under influence of sewage treatment plant discharges. Mutat Res 491(1):39–44

    Article  CAS  Google Scholar 

  • Grisolia CK, Rivero CLG, Starling FLRM, da Silva ICR, Barbosa AC, Dore JG (2009) Profile of micronucleus frequencies and DNA damage in different species of fish in a eutrophic tropical lake. Genet Mol Biol 32(1):138–143

    Article  CAS  Google Scholar 

  • Guillén D, Ginebreda A, Farré M, Darbra RM, Petrovic M, Gros M et al (2012) Prioritization of chemicals in the aquatic environment based on risk assessment: analytical, modeling and regulatory perspective. Sci Total Environ 440:236–252

    Article  Google Scholar 

  • Heddle JA, Hite M, Kirkhart B, Mavournin K, MacGregor JT, Newell GW et al (1983) The induction of micronuclei as a measure of genotoxicity. Mutat Res 123:61–118

    Article  CAS  Google Scholar 

  • Karr JR (1981) Assessment of biotic integrity using fish communities. Fisheries 6(6):21–27

    Article  Google Scholar 

  • Kominoski JS, Rosemond AD (2012) Conservation from the bottom up: forecasting effects of global change on dynamics of organic matter and management needs for river networks. Freshw Sci 31(1):51–68

    Article  Google Scholar 

  • Matsumoto FE, Cólus IMS (2000) Micronucleus frequencies in Astyanax bimaculatus (Characidae) treated with cyclophosphamide or vinblastine sulfate. Genet Mol Biol 23(2):489–492

    Article  CAS  Google Scholar 

  • Miao M, Fu R, Yang D, Zheng L (1998) Vicia root micronucleus assay on the clastogenicity of the water samples from the Xiaoqing River in Shandong Province of the People’s Republic of China. Mutat Res 426:143–145

    Article  Google Scholar 

  • Monserrat JM, Martinez PE, Geracitano LA, Amado LL, Martins CMG, Pinho GLL et al (2007) Pollution biomarkers in estuarine animals: critical review and new perspectives. Toxicol Pharm 146:221–234

    Google Scholar 

  • Moraes DS, Jordão BQ (2002) Degradação de recursos hídricos e seus efeitos sobre a saúde humana. Rev Saúde Públ 36(3):370–374

    Article  Google Scholar 

  • Nascimento WM, Villaça MG (2008) Bacias hidrográficas: planejamento e gerenciamento. Revista Eletrônica de Geógrafos Brasileiros 7:102–121

    Google Scholar 

  • Ohe T, Watanabe T, Wakabayashi K (2004) Mutagens in the surface water: a review. Mutat Res 567:109–149

    Article  CAS  Google Scholar 

  • Ossana NA, Castañé PM, Salibián A (2013) Use of Lithobates catesbeianus tadpoles in a multiple biomarker approach for the assessment of water quality of the Reconquista river (Argentina). Arch Environ Contam Toxicol 65:486–497

    Article  CAS  Google Scholar 

  • Pantaleão SM, Alcântara AV, Alvez JPH, Spanó MA (2006) The piscine micronucleus test to assess the impact of pollution on the Japaratuba river in Brazil. Environ Mol Mutagen 47(3):219–224

    Article  Google Scholar 

  • Pillon CN, Miranda CR, Guidoni AL, Cordebella A, Pereira RK (2003) Diagnóstico das propriedades suinícolas da área de abrangência do Consórcio Lambari, SC. Consórcio Lambari. http://www.consorciolambari.com.br/index.php?option=com_phocadownload&view=category&download=4:diagnosticos-propriedades84&id=1:tac&Itemid=43. Accessed 14 Jun 2012

  • Porto MFA, Porto RLL (2008) Gestão de bacias hidrográficas. Estudos Avançados 22(63):43–60

    Article  Google Scholar 

  • Postel SL, Daily GC, Ehrlich PR (1996) Human appropriation of renewable fresh water. Science 271:785–787

    Article  CAS  Google Scholar 

  • Rashed MN (2001) Monitoring of environmental heavy metals in fish from Nasser Lake. Environ Int 27(1):27–33

    Article  CAS  Google Scholar 

  • Rivero CLG (2007) Perfil da frequência de micronúcleos e de danos no DNA de diferentes espécies de peixes do Lago Paranoá, Brasília-DF, Brasil. Dissertation, University of Brasília, Brasília, Brazil

  • Rocha CAM, Gomes CDF, Ribeiro RFG Jr, Pinheiro PHS (2011) Detection of micronuclei and other nuclear abnormalities in Oreochromis niloticus exposed to potassium dichromate. Glob Vet 7(3):301–304

    Google Scholar 

  • Rodrigues FAC (2005) Ecogenotoxicologia dos agrotóxicos: Avaliação comparativa entre ecossistema agrícola e área de proteção ambiental. Dissertation, University of Brasília, Brasíli, Brazil

  • Scott AJ, Knott M (1974) A cluster analysis method for grouping means in the analysis of variance. Biometrics 30:507–512

    Article  Google Scholar 

  • Silva AC, Nepomuceno JC (2010) Avaliação da frequência de micronúcleos em eritrócitos periféricos de mandi-amarelo (Pimelodus maculatus) do rio Paranaíba. Perquirère 7(1):167–179

    Google Scholar 

  • Silva AEP, Angelis CF, Machado LAT, Waichaman AV (2008) Influência da precipitação na qualidade da água do Rio Purus. Acta Amazonica 38(4):733–742

    Article  CAS  Google Scholar 

  • Silva RRP, Pires OR Jr, Grisolia CK (2010) Toxicity and genotoxicity in Astyanax bimaculatus (Characidae) induced by microcystins from a bloom of Microcystis spp. Genet Mol Biol 33(4):750–755

    Article  Google Scholar 

  • Steinmetz RLR, Kunz A, Dressler FEMM, Martin AF (2009) Study of metal distribution in raw end screened swine manure. Clean Soil Air Water 37(3):239–244

    Article  CAS  Google Scholar 

  • Tan F, Wang M, Wang W, Lu Y (2008) Comparative evaluation of the cytotoxicity sensitivity of six fish cell lines to four heavy metals in vitro. Toxicol In Vitro 22:164–170

    Article  CAS  Google Scholar 

  • R Core Team (2013) R: a language and environment for statistical computing. R Foundation for Statistical Computing, Vienna. http://www.R-project.org/. Accessed 18 June 2013

  • Techio VH, Stolberg J, Kunz A, Zanin E, Perdomo CC (2011) Genotoxicity of swine effluents. Water Sci Technol 971:970–976

    Google Scholar 

  • Vanzella TP, Martinez CBR, Cólus IMS (2007) Genotoxic and mutagenic effects of diesel oil water fraction on a neotropical fish species. Mutat Res 631:36–43

    Article  CAS  Google Scholar 

  • Veiga MM, Silva DM, Veiga LBE, Fari MVC (2006) Análise da contaminação dos sistemas hídricos por agrotóxicos numa pequena comunidade rural do Sudeste do Brasil. Cad Saúde Pública 22(11):2391–2399

    Article  Google Scholar 

  • Vine MF (1990) Micronuclei. In: Hulka BS, Wilcosky TC, Griffith JD (eds) Biological markers in epidemiology. Oxford University, New York, pp 125–146

    Google Scholar 

  • Witters HE (1998) Chemical speciation dynamics and toxicity assessment in aquatic ecosystems. Ecotox Environ Safe 41:90–95

    Article  CAS  Google Scholar 

  • World Meteorological Organization (1992) The Dublin Statement and Report of the Conference. In: International conference on water and the environment, development issues for the 21st century, Dublin

  • Zar JH (1999) Biostatistical analysis, 4ª edn. Prentice-Hall, Upper Saddle River

    Google Scholar 

Download references

Acknowledgments

We thank the Grupo de Estudos e Pesquisas em Meio Ambiente (Group of Studies and Research in the Environment), the Government of the Santa Catarina State for scholarship, and to Dr. Elisete A. Barp for support.

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Authors and Affiliations

  1. Departamento de Ecologia e Zoologia, Universidade Federal de Santa Catarina (UFSC), Campus Universitário Reitor João David Ferreira Lima, Trindade, Florianópolis, SC, CEP 88040-970, Brazil

    Juliano André Bogoni & Neide Armiliato

  2. Grupo de Estudos e Pesquisas em Meio Ambiente, Universidade do Contestado, Campus Concórdia, Rua Victor Sopelsa, 3000, Bairro Salete, Concórdia, SC, CEP 89700-000, Brazil

    Juliano André Bogoni, Neide Armiliato & Celi Teresinha Araldi-Favassa

  3. Departamento de Biologia, Laboratório de Citogenética, Universidade Federal de Lavras, Lavras, MG, CEP 37200-000, Brazil

    Vânia Helena Techio

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  1. Juliano André Bogoni
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  2. Neide Armiliato
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Correspondence to Vânia Helena Techio.

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Bogoni, J.A., Armiliato, N., Araldi-Favassa, C.T. et al. Genotoxicity in Astyanax bimaculatus (Twospot Astyanax) Exposed to the Waters of Engano River (Brazil) as Determined by Micronucleus Tests in Erythrocytes. Arch Environ Contam Toxicol 66, 441–449 (2014). https://doi.org/10.1007/s00244-013-9990-5

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