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Biologic Responses of Bacteria Communities Living at the Mucus Secretion of Common Carp (Cyprinus carpio) After Exposure to the Carbon Nanomaterial Fullerene (C60)

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Abstract

Bacteria communities living in mucus secretions of common carp Cyprinus carpio (Cyprinidae) were exposed to the organic nanomaterial fullerene (C60) to evaluate its potential bactericidal effects. End points analyzed were viability, growth, reactive oxygen species (ROS) concentration, and total antioxidant competence against peroxyl radicals. Viability was not affected (p > 0.05), whereas growth was arrested (p < 0.05) after 3 hours of exposure to the three concentration of C60 assayed (0.1, 1, and 10 mg/L). Levels of RO measured at different C60 concentration showed that some colonies were reactive (significant dose-response relation, p < 0.05) to C60, whereas others were not. The nonreactive colonies to C60 presented higher antioxidant competence to peroxyl radicals compared with the reactive colonies (p < 0.05). The strains isolated and identified by polymerase chain reaction (PCR) products of 16S rRNA showed a predominance of Aeromonas genus between all the isolated Gram-negative bacteria. Thus, the present results indicate that C60 affects bacterial communities that live in mucus secretions of common carp.

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Acknowledgments

The research was supported by a grant from the Brazilian agency CNPq (Proc. nº 471459/2007-0) given to J. M. Monserrat and by a grant from the LASPAU/Fincyt Peruvian Research Fund given to R. Elias Letts. J. M. Monserrat and M. R. Bogo are research fellows from CNPq. The authors are indebted to J. L. Ribas Ferreira for invaluable logistic support and suggestions and to the Electronic Microscopy Center at the Universidade Federal do Rio Grande do Sul The technical aid from S. Carvalho Rodrigues was also extremely important during the execution of this study. The authors also acknowledge the suggestions and corrections of an anonymous reviewer.

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Authors and Affiliations

  1. Programa de Pós-Graduação em Ciências Fisiológicas—Fisiologia Animal Comparada, Universidade Federal de Rio Grande, Rio Grande, Brazil

    Rafaela Elias Letts & José M. Monserrat

  2. Universidad Peruana Cayetano Heredia, Lima, Peru

    Rafaela Elias Letts

  3. Laboratório de Biologia Genômica e Molecular, Faculdade de Biociências, Pontifícia Universidade Católica do Rio Grande do Sul, Porto Alegre, Brazil

    Talita C. B. Pereira & Mauricio Reis Bogo

  4. Instituto Nacional de Ciência e Tecnologia Translacional em Medicina, Porto Alegre, Brazil

    Mauricio Reis Bogo

  5. Instituto de Ciências Biológicas, Universidade Federal do Rio Grande, Rio Grande, Brazil

    José M. Monserrat

  6. Instituto Nacional de Ciência e Tecnologia de Nanomateriais de Carbono, Rio Grande, Brazil

    José M. Monserrat

Authors
  1. Rafaela Elias Letts
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  2. Talita C. B. Pereira
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  3. Mauricio Reis Bogo
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  4. José M. Monserrat
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Correspondence to José M. Monserrat.

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Letts, R.E., Pereira, T.C.B., Bogo, M.R. et al. Biologic Responses of Bacteria Communities Living at the Mucus Secretion of Common Carp (Cyprinus carpio) After Exposure to the Carbon Nanomaterial Fullerene (C60). Arch Environ Contam Toxicol 61, 311–317 (2011). https://doi.org/10.1007/s00244-010-9618-y

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  • DOI: https://doi.org/10.1007/s00244-010-9618-y

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