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Bioassessment of an Appalachian Headwater Stream Influenced by an Abandoned Arsenic Mine

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Abstract

Recent debate concerning the modification of safe drinking water standards for arsenic (As) has led to increased awareness of the risks As poses to both humans and the environment. However, few studies have examined the effects of As on the diversity and composition of aquatic assemblages in streams. Benthic macroinvertebrate surveys, chemical analysis of water column and sediment, and laboratory toxicity tests were conducted to assess effects of an abandoned As mine on a headwater stream, and to determine the primary component of toxicity. The average 48-hr LC50 value for Daphnia magna was 4316 μg As/L, and the average 96-hr LC50 value for Lepidostoma spp. was 2138 μg As/L. Reproduction was significantly reduced for D. magna at concentrations ≥312 μg As/L in water column laboratory bioassays, and for treatments in bioassays with sediments containing elevated As (≥2630 mg/kg). These results support the findings of the in-stream benthic macroinvertebrate survey as the density and percent Ephemeroptera + Plecoptera, + Trichoptera (EPT) were substantially lower at sites downstream of the mine compared to upstream reference sites. Results of bioassays comparing the toxicity of As-contaminated site water and upstream reference water spiked with As salts suggest that As is the primary component of toxicity impacting the stream. Measured As concentrations at downstream sites were above the recommended Criterion Maximum Concentration of 340 μg As/L and Criterion Continuous Concentration of 150 μg As/L for protection of aquatic life published by the United States Environmental Protection Agency. At the study site, elevated As concentrations likely prevent recruitment of benthic macroinvertebrates and recovery of the perturbed headwater stream.

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Acknowledgments

We thank the Virginia Tech Stream Team, Aquatic Ecotoxicology, and Hydrogeosciences labs for assistance at various times throughout this research. This work was supported by NSF award EAR-0207784.

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Authors and Affiliations

  1. Aquatic Ecotoxicology Lab. Department of Biology, Virginia Polytechnic Institute and State University, Blacksburg, Virginia, 24061, USA

    Theodore W. Valenti & Donald S. Cherry

  2. Stream Ecology Lab. Department of Biology, Virginia Polytechnic Institute and State University, Blacksburg, Virginia, 24061, USA

    Jake L. Chaffin, H. Maurice Valett & Megan Charles

  3. Department of Geosciences, Virginia Polytechnic Institute and State University, Blacksburg, Virginia, 24061, USA

    Madeline E. Schreiber

Authors
  1. Theodore W. Valenti
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  2. Jake L. Chaffin
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  3. Donald S. Cherry
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  4. Madeline E. Schreiber
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  5. H. Maurice Valett
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  6. Megan Charles
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Correspondence to Theodore W. Valenti.

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Valenti, T.W., Chaffin, J.L., Cherry, D.S. et al. Bioassessment of an Appalachian Headwater Stream Influenced by an Abandoned Arsenic Mine. Arch Environ Contam Toxicol 49, 488–496 (2005). https://doi.org/10.1007/s00244-004-0222-x

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  • DOI: https://doi.org/10.1007/s00244-004-0222-x

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