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The low levels of circulating hepatocyte growth factor in nephrolithiasis cases: independent from gene polymorphism

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Abstract

Environmental and genetic factors are important in development of nephrolithiasis. In a recent study, it has been demonstrated that hepatocyte growth factor (HGF) has an anti-apoptotic effect and thus can reduce the adhesion of calcium oxalate monohydrate crystals to renal epithelial cells. The aim of this study was to evaluate the HGF serum levels and its two gene polymorphisms and possible association of the two in patients with nephrolithiasis. One hundred and five patients with nephrolithiasis and 70 healthy volunteers with similar demographic features were included in this study. Serum HGF levels were measured, and HGF intron 13 C>A (in 102 stone patients and 68 healthy subjects) and intron 14 T>C (in 99 stone patients and 56 healthy subjects) polymorphisms were determined using real-time polymerase chain reaction with TaqMan allelic discrimination method. There were no statistically significant differences in HGF intron 13 C>A and intron 14 T>C polymorphisms between the control and patient groups (X 2 = 1.72 df = 2; p = 0.42, and X 2 = 0.68 df = 2; p = 0.71, respectively). Mean serum HGF concentration was significantly lower in the stone disease patients than in the control subjects (1.05 ± 0.63 pg/mL and 1.35 ± 0.58 ng/mL respectively, p = 0.0001). When allele distribution frequency between stone patients and healthy subjects was compared, there were no significant differences in intron 13 and intron 14 allele distributions between two groups (p = 0.43 and p = 0.44, respectively). It may be concluded from the findings that decrease in HGF levels may play a role in renal stone formation, independent from gene polymorphisms.

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Abbreviations

COM:

Calcium oxalate monohydrate

HGF:

Hepatocyte growth factor

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Acknowledgments

This study was financially supported by a research fund from the Scientific and Technological Research Council of Turkey (TUBITAK) (Project number: 107S273). This study has been accepted as a poster presentation in the “American Association for Clinical Chemistry (AACC) Annual Meeting on July 25–29, 2010, California, USA”.

Conflict of interest

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Department of Biochemistry, School of Medicine, Ataturk University, 25240, Erzurum, Turkey

    Nurinnisa Ozturk, Hulya Aksoy, Abdulkadir Yildirim & Fatih Akcay

  2. Department of Urology, School of Medicine, Ataturk University, Erzurum, Turkey

    Yilmaz Aksoy

  3. Kocaeli Karamursel State Hospital, Karamursel, Kocaeli, Turkey

    Vefa Yanmaz

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  1. Nurinnisa Ozturk
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  2. Hulya Aksoy
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  3. Yilmaz Aksoy
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  4. Abdulkadir Yildirim
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  5. Fatih Akcay
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  6. Vefa Yanmaz
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Correspondence to Nurinnisa Ozturk.

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Ozturk, N., Aksoy, H., Aksoy, Y. et al. The low levels of circulating hepatocyte growth factor in nephrolithiasis cases: independent from gene polymorphism. Urolithiasis 43, 427–432 (2015). https://doi.org/10.1007/s00240-015-0793-1

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  • DOI: https://doi.org/10.1007/s00240-015-0793-1

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