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Inhibition of adherence of multi-drug resistant E. coli by proanthocyanidin

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Abstract

Proanthocyanidin is commonly used for inhibiting urinary tract infection (UTI) of sensitive strains of Escherichia coli. The aim of this study was to investigate the effect of proanthocyanidin on adherence of uropathogenic multi-drug resistant E. coli to uroepithelial cells, which has not yet been investigated so far. Extracts of the purified proanthocyanidin were prepared from dried cranberry juice. Purity and structural assignment of proanthocyanidin was assessed using high performance liquid chromatography and 13C nuclear magnetic resonance spectroscopy, respectively. Subsequently, its affect on multi-drug resistant bacteria as well as quantification of anti-adherence bioactivity on human vaginal and bladder epithelial cells was appraised. Inhibition of adherence to an extent of about 70% with multi-drug resistant E. coli strains was observed on uroepithelial cell. The anti-adherence bioactivity of the proanthocyanidin was detected at concentrations of 10–50 µg/ml with significant bacteriuria. Probable proanthocyanidin through A-type linkages either combines to P-fimbriae of bacterial cells or modifies the structural entity of P-fimbriae and inhibits bacterial adherence to uroepithelial cells. The proanthocyanidin exhibited anti-adherence property with multi-drug resistant strains of uropathogenic P-fimbriated E. coli with in vitro study. Hence proanthocyanidin may be considered as an inhibitory agent for multi-drug resistant strains of E. coli adherence to uroepithelial cells.

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Abbreviations

NMR:

Nuclear magnetic resonance

HPLC:

High performance liquid chromatography

RBCs:

Red blood cells

E. coli :

Escherichia coli

VECs:

Vaginal epithelial cells

BECs:

Bladder epithelial cells

MF:

McFarland

CFA:

Colonization factor agar

AMC:

Amoxicillin–Clavulanic Acid

UTI:

Urinary tract infection

PBS:

Phosphate buffer saline solution

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Acknowledgments

We gratefully acknowledge the financial support from the Department of Science and Technology and council of scientific and industrial research, New Delhi, India.

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None.

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Authors and Affiliations

  1. Centre of Biomedical Magnetic Resonance, Sanjay Gandhi Postgraduate Institute of Medical Sciences, Raebareli Road, Lucknow, 226 014, India

    Ashish Gupta, G. A. Nagana Gowda & Chunni Lal Khetrapal

  2. Department of Microbiology, Lady Hardinge Medical College, New Delhi, India

    Mayank Dwivedi

  3. Department of Biochemistry, Chhatrapati Shahuji Maharaj Medical University, Lucknow, 226003, India

    Abbas Ali Mahdi

  4. Vattikuti Urology Institute, Henry Ford Hospital System, Detroit, MI, USA

    Mahendra Bhandari

Authors
  1. Ashish Gupta
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  2. Mayank Dwivedi
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  3. Abbas Ali Mahdi
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  4. G. A. Nagana Gowda
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  5. Chunni Lal Khetrapal
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  6. Mahendra Bhandari
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Correspondence to Ashish Gupta.

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Gupta, A., Dwivedi, M., Mahdi, A.A. et al. Inhibition of adherence of multi-drug resistant E. coli by proanthocyanidin. Urol Res 40, 143–150 (2012). https://doi.org/10.1007/s00240-011-0398-2

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  • DOI: https://doi.org/10.1007/s00240-011-0398-2

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