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The efficacy of a novel antibacterial hydroxyapatite nanoparticle-coated indwelling urinary catheter in preventing biofilm formation and catheter-associated urinary tract infection in rabbits

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Abstract

The aim of this study was to investigate of the efficacy and reliability of a novel antimicrobial hydroxyapatite (HA) nanoparticle coating of urethral catheters, in the prophylaxis of biofilm formation and bacteriuria in rabbits. A total of 60 male rabbits were randomized to the control and study groups and each group was divided into three subgroups depending on 3, 5 and 7 days of the urethral catheterization period. The rabbits in the study group were catheterized with Ag+-incorporated nano-HA coated urethral catheters and those in the control group with standard silicon–latex urethral catheters. Urine and catheter surface smear samples were conducted for bacteriological analysis. Catheter cross-section samples were undergone measuring of biofilm thickness. Tissue samples of bladder and urethra were inspected for histological changes. The results indicate that at the end of 7 days of the catheterization period, the number of the rabbits with bacteriuria was significantly lower in the study group versus control group (p  = 0.020). The biofilm formation on luminal surface of the catheters was significantly thinner in the study group versus control group, at the end of 5 and 7 days of the catheterization period (0.035 and 0.035, respectively).No histological adverse change or particle penetration was detected in the urothelium. In conclusion, it was observed that Ag+ + HA nanoparticle coating significantly lowered the incidence of catheter-related bacteriuria and decreased biofilm formation, at the end of 7 days study period. The novel antimicrobial urethral catheter coating appeared to have a potential in the prophylaxis of catheter-induced urinary tract infections.

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Abbreviations

SEM:

Scanning electron microscopy

EDX:

Energy dispersive X-ray spectrophotometer

MTT:

3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide

HA:

Hydroxyapatite

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Acknowledgment

Kenan Dayıoğlu Vet. Dr provided the rabbits and assisted with their care in Çukurova University Medical Sciences Experimental Research and Application Center. İlker Ünal MSc provided statistical collation of the data.

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Authors and Affiliations

  1. Department of Urology, Adana Numune Training and Research Hospital, Adana, Turkey

    Yalçın Evliyaoğlu

  2. Şırnak State Hospital, Sirnak, Turkey

    Murat Kobaner

  3. Department of Chemical Engineering, Anadolu University, Eskisehir, Turkey

    Hande Çelebi

  4. Viranşehir State Hospital, Viransehir, Urfa, Turkey

    Kazım Yelsel

  5. Material Science and Engineering Department, Engineering and Architecture Faculty, Anadolu University, Eskisehir, Turkey

    Aydın Doğan

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  1. Yalçın Evliyaoğlu
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  2. Murat Kobaner
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  3. Hande Çelebi
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  4. Kazım Yelsel
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  5. Aydın Doğan
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Correspondence to Yalçın Evliyaoğlu.

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Evliyaoğlu, Y., Kobaner, M., Çelebi, H. et al. The efficacy of a novel antibacterial hydroxyapatite nanoparticle-coated indwelling urinary catheter in preventing biofilm formation and catheter-associated urinary tract infection in rabbits. Urol Res 39, 443–449 (2011). https://doi.org/10.1007/s00240-011-0379-5

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  • DOI: https://doi.org/10.1007/s00240-011-0379-5

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