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Association of vitamin d receptor genotypes with calcium excretion in nephrolithiatic subjects in northern India

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Abstract

Our objective was to investigate the association between the vitamin D receptor (VDR) allelic variants (Bsm I and Fok I) and nephrolithiasis in northern India. A total of 150 nephrolithiatic patients and 100 age and sex matched controls were enrolled for study. A 10 ml blood sample was obtained for biochemical analysis and DNA isolation. In addition, 24 h urine samples were obtained from each patient for the estimation of calcium and creatinine. PCR was performed for the Bsm I and Fok I VDR variants. The association between Bsm I and Fok I VDR polymorphism and nephrolithiasis was investigated after digestion with restriction enzymes (3 U). The product was analysed on 3% agarose gel for Bsm I and 15% polyacrylamide gel for Fok I allelic variants. We did not observe any significant differences in the prevalence of either the Bsm I or Fok I VDR genotypes between stone formers and controls. The B allele was found to be more prevalent in hypercalciuric patients compared to controls and nephrolithiatic subjects. The subjects with the bb genotype exhibited a higher calcium excretion than the BB genotype. Patients with the F allele were also found to excrete higher urinary calcium. VDR genotypes may be associated with increased calcium excretion in hypercalciuric nephrolithiatic subjects.

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Authors and Affiliations

  1. Department of Experimental Medicine and Biotechnology, Postgraduate Institute of Medical Education and Research, Chandigarh 160012, India

    Vandana Relan & Madhu Khullar

  2. Department of Urology, Postgraduate Institute of Medical Education and Research, Chandigarh 160012, India

    S. K. Singh & S. K. Sharma

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  1. Vandana Relan
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  2. Madhu Khullar
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  3. S. K. Singh
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  4. S. K. Sharma
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Correspondence to Madhu Khullar.

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Relan, V., Khullar, M., Singh, S.K. et al. Association of vitamin d receptor genotypes with calcium excretion in nephrolithiatic subjects in northern India. Urol Res 32, 236–240 (2004). https://doi.org/10.1007/s00240-004-0414-x

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  • DOI: https://doi.org/10.1007/s00240-004-0414-x

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