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Expression of functional protease-activated receptor 1 in human prostate cancer cell lines

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Abstract

Functional protease-activated receptors (PAR) are expressed by a variety of malignant cells. In the present study, RT-PCR assays demonstrated the expression of the thrombin receptor PAR-1 mRNA in human prostate cancer cell lines DU 145, LnCAP, and SV40-immortalized human prostate epithelial cell line PNT1A. In contrast, the additional thrombin receptors PAR-3 and PAR-4 were not detected. PAR-1 protein localized to the cellular surface was detected by flow cytometry in all three cell lines. To demonstrate the functional importance of the PAR-1, the effects of different concentrations of thrombin on cell proliferation kinetics were assessed. The treatment of growth-arrested cells with varying concentrations of thrombin demonstrated dose- and time-dependent effects. At low concentration (<0.5 U/ml), thrombin induced proliferation of all prostate-derived cell lines. Thrombin at higher concentration (1.0 U/ml) initially stimulated PNT1A and LnCAP cells to proliferate (time of thrombin application 24 h and 48 h) followed by inhibited growth when assessed after 72 h of incubation. In contrast, 1.0 U/ml thrombin caused earlier inhibition of DU 145 proliferation starting at 48 h of incubation. Our results suggest that PAR-1 mediates the proliferation-modulating effects of thrombin on prostate cancer cells.

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Acknowledgements

The excellent technical assistance provided by laboratory staff members is gratefully acknowledged. This work was supported by grant GRK 19/3-02 from the Deutsche Forschungsgemeinschaft (Bonn, Germany).

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  1. University of Rostock, Medical Faculty, Institute of Clinical Chemistry and Pathobiochemistry, Ernst-Heydemann-Strasse 6, 18057 Rostock, Germany

    Jian Liu, Manuela Bastian, Peter Kohlschein, Peter Schuff-Werner & Michael Steiner

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  1. Jian Liu
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  2. Manuela Bastian
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  3. Peter Kohlschein
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  5. Michael Steiner
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Correspondence to Michael Steiner.

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Liu, J., Bastian, M., Kohlschein, P. et al. Expression of functional protease-activated receptor 1 in human prostate cancer cell lines. Urol Res 31, 163–168 (2003). https://doi.org/10.1007/s00240-003-0309-2

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