Abstract
A long-range exploration of expression levels through wide chromosome territories was carried out in three species (pig, cattle, and chicken) by aligning EST counts against the human genome. This strategy made it possible to produce expression profiles that were very similar between pig and cattle and that were significantly correlated with chicken levels of expression. In parallel with these alignments, we developed a statistical approach enabling us to screen genomic regions for both underexpression and overexpression at the chromosome level within a given species, as well as interspecifically. The observed correlations are indicative of the existence of interspecifically conserved domains of gene expression, not only for housekeeping genes (which are highly expressed), but also for regions where genes are significantly underexpressed. Furthermore, our strategy made it possible to point out regions that are differentially regulated between species. These expression data were crossed with available comparative mapping information for pigs and cattle, suggesting that coregulated regions are syntenic in various mammals.
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References
MR Band JH Larson M Rebeiz CA Green DW Heyen J Donovan R Windish C Steining P Mahyuddin JE Womack HA Lewin (2000) ArticleTitleAn ordered comparative map of the cattle and human genomes Genome Res 10 IssueID9 1359–1368 Occurrence Handle10.1101/gr.145900 Occurrence Handle1:CAS:528:DC%2BD3cXms1yjur4%3D Occurrence Handle10984454
CE Bishop DJ Whitworth Y Qin AI Agoulnik IU Agoulnik WR Harrison RR Behringer PA Overbeek (2000) ArticleTitleA transgenic insertion upstream of Sox9 is associated with dominant XX sex reversal in the mouse Nat Genet 26 IssueID4 490–494 Occurrence Handle10.1038/82652 Occurrence Handle1:CAS:528:DC%2BD3cXptVWgt7o%3D Occurrence Handle11101852
JM Boss PE Jensen (2003) ArticleTitleTranscriptional regulation of the MHC class II antigen presentation pathway Curr Opin Immunol 15 IssueID1 105–111 Occurrence Handle10.1016/S0952-7915(02)00015-8 Occurrence Handle1:CAS:528:DC%2BD38XpsFegsbk%3D Occurrence Handle12495741
AM Boutanaev AI Kalmykova YY Shevelyov DI Nurminsky (2002) ArticleTitleLarge clusters of co-expressed genes in the Drosophila genome Nature 420 IssueID6916 666–669 Occurrence Handle10.1038/nature01216 Occurrence Handle1:CAS:528:DC%2BD38XpsVSitbk%3D Occurrence Handle12478293
H Caron B Schaik ParticleVan M Mee ParticleVan Der F Baas G Riggins P Sluis ParticleVan MC Hermus R Asperen ParticleVan K Boon PA Voute S Heisterkamp A Kampen ParticleVan R Versteeg (2001) ArticleTitleThe human transcriptome map: Clustering of highly expressed genes in chromosomal domains Science 291 IssueID5507 1289–1292 Occurrence Handle10.1126/science.1056794 Occurrence Handle1:CAS:528:DC%2BD3MXhtlSgs70%3D Occurrence Handle11181992
D Carter L Chakalova CS Osborne YF Dai P Fraser (2002) ArticleTitleLong-range chromatin regulatory interactions in vivo Nat Genet 32 IssueID4 623–626 Occurrence Handle10.1038/ng1051 Occurrence Handle1:CAS:528:DC%2BD38XptVShu74%3D Occurrence Handle12426570
C Charlier K Segers L Karim T Shay G Gyapay N Cockett M Georges (2001) ArticleTitleThe callipyge mutation enhances the expression of coregulated imprinted genesin cis without affecting their imprinting status Nat Genet 27 IssueID4 367–369 Occurrence Handle10.1038/86856 Occurrence Handle1:CAS:528:DC%2BD3MXjtFShs7w%3D Occurrence Handle11279514
J Cocquet E Pailhoux F Jaubert N Servel X Xia M Pannetier E Baere ParticleDe L Messiaen C Cotinot M Fellous RA Veitia (2002) ArticleTitleEvolution and expression of FOXL2 J Med Genet 39 IssueID12 916–921 Occurrence Handle10.1136/jmg.39.12.916 Occurrence Handle1:CAS:528:DC%2BD3sXlsFOntg%3D%3D Occurrence Handle12471206
T Cremer C Cremer (2001) ArticleTitleChromosome territories, nuclear architecture and gene regulation in mammalian cells Nat Rev Genet 2 IssueID4 292–301 Occurrence Handle10.1038/35066075 Occurrence Handle1:CAS:528:DC%2BD3MXisVCqurc%3D Occurrence Handle11283701
T Cremer A Kurz R Zirbel S Dietzel B Rinke E Schrock Mr Speicher U Mathieu A Jauch P Emmerich et al. (1993) ArticleTitleRole of chromosome territories in the functional compartmentalization of the cell nucleus Cold Spring Harb Symp Quant Biol 58 777–792 Occurrence Handle1:CAS:528:DyaK2MXmtF2rtQ%3D%3D Occurrence Handle7525149
S Ferdinandusse S Denis PT Clayton A Graham JE Rees JT Alien BN McLean AY Brown P Vreken HR Waterham RJ Wanders (2000) ArticleTitleMutations in the gene encoding peroxisomal alpha-methylacyl-CoA racemase cause adult-onset sensory motor neuropathy Nat Genet 24 IssueID2 188–191 Occurrence Handle10.1038/72861 Occurrence Handle1:CAS:528:DC%2BD3cXhtFCgtro%3D Occurrence Handle10655068
N Fujishima-Kanaya D Toki K Suzuki T Sawazaki H Hiraiwa M Iida T Hayashi H Uenishi Y Wada Y Ito Awata (2003) ArticleTitleDevelopment of 50 gene-associated microsatellite markers using BAC clones and the construction of a linkage map of swine chromosome 4 Anim Genet 34 IssueID2 135–141 Occurrence Handle10.1046/j.1365-2052.2003.00967.x Occurrence Handle1:CAS:528:DC%2BD3sXjtlKnt78%3D Occurrence Handle12648097
DG Jennen RP Crooijmans B Kamps R Acar JJ Poel Particlevan der MA Groenen (2003) ArticleTitleComparative map between chicken chromosome 15 and human chromosomal region 12q24 and 22q11-q12 Mamm Genome 14 IssueID9 629–639 Occurrence Handle10.1007/s00335-003-3007-z Occurrence Handle1:CAS:528:DC%2BD3sXnslGmsr0%3D Occurrence Handle14629113
A Kurz S Lampel Je Nickolenko J Bradl A Benner RM Zirbel T Cremer P Lichter (1996) ArticleTitleActive and inactive genes localize preferentially in the periphery of chromosome territories J Cell Biol 135 IssueID5 1195–1205 Occurrence Handle10.1083/jcb.135.5.1195 Occurrence Handle1:CAS:528:DyaK28XntlamtL4%3D Occurrence Handle8947544
DM Larkin A Everts-van der Wind M Rebeiz PA Schweitzer S Bachman C Green CL Wright EJ Campos LD Benson J Edwards L Liu K Osoegawa JE Womack PJ Jong Particlede HA Lewin (2003) ArticleTitleA cattle-human comparative map built with cattle BAC-ends and human genome sequence Genome Res 13 IssueID8 1966–1972 Occurrence Handle12902387
JG Lawrence (2002) ArticleTitleShared strategies in gene organization among prokaryotes and eukaryotes Cell 23 IssueID110(4) 407–413 Occurrence Handle10.1016/S0092-8674(02)00900-5
MJ Lercher AO Urrutia LD Hurst (2002) ArticleTitleClustering of housekeeping genes provides a unified model of gene order in the human genome Nat Genet 131 IssueID2 180–183 Occurrence Handle10.1038/ng887
L Liu G Gong Y Liu S Natarajan DM Larkin A Everts-van der Wind M Rebeiz JE Beever (2004) ArticleTitleMulti-species comparative mapping in silico using the COMPASS strategy Bioinformatics 22 IssueID20(2) 148–154 Occurrence Handle10.1093/bioinformatics/bth018
KA Loffler D Zarkower P Koopman (2003) ArticleTitleEtiology of ovarian failure in blepharophimosis ptosis epicanthus in versus syndrome: FOXL2 is a conserved, early-acting gene in vertebrate ovarian development Endocrinology 144 IssueID7 3237–3243 Occurrence Handle10.1210/en.2002-0095 Occurrence Handle1:CAS:528:DC%2BD3sXkvF2ktbY%3D Occurrence Handle12810580
NL Mahy PE Perry WA Bickmore (2002a) ArticleTitleGene density and transcription influence the localization of chromatin outside of chromosome territories detectable by FISH J Cell Biol 159 IssueID5 753–763 Occurrence Handle10.1083/jcb.200207115 Occurrence Handle1:CAS:528:DC%2BD38XpsFCqtbo%3D
NL Mahy PE Perry S Gilchrist RA Baldock WA Bickmore (2002b) ArticleTitleSpatial organization of active and inactive genes and non-coding DNA within chromosome territories J Cell Biol 157 IssueID4 579–589 Occurrence Handle10.1083/jcb.200111071 Occurrence Handle1:CAS:528:DC%2BD38XjslGlsL0%3D
E Pailhoux B Vigier S Chaffaux N Servel S Taourit Jp Furet M Fellous F Grosclaude Ep Cribiu C Cotinot D Vaiman (2001) ArticleTitleA 11.7-kb deletion triggers intersexuality and polledness in goats Nat Genet 29 IssueID4 453–458 Occurrence Handle10.1038/ng769 Occurrence Handle1:CAS:528:DC%2BD3MXovFCrsro%3D Occurrence Handle11726932
L Parada T Misteli (2002) ArticleTitleChromosome positioning in the interphase nucleus Trends Cell Biol 12 IssueID9 425–432 Occurrence Handle10.1016/S0962-8924(02)02351-6 Occurrence Handle1:CAS:528:DC%2BD38XmvVWlt70%3D Occurrence Handle12220863
CS Raymond CE Shamu MM Shen KJ Seifert B Hirsch J Hodgkin D Zarkower (1998) ArticleTitleEvidence for evolutionary conservation of sex-determining genes Nature 391 IssueID6668 691–695 Occurrence Handle10.1038/35618 Occurrence Handle1:CAS:528:DyaK1cXht1GmtLw%3D Occurrence Handle9490411
M Rebeiz HA Lewin (2000) ArticleTitleCompass of 47,787 cattle ESTs Anim Biotechnol 11 IssueID2 75–241 Occurrence Handle1:CAS:528:DC%2BD3cXlvFyhsL4%3D Occurrence Handle10953882
A Reymond V Marigo MB Yaylaoglu A Leoni C Ucla N Scamuffa C Caccioppoli ET Dermitzakis R Lyle S Banfi G Eichele SE Antonarakis A Ballabio (2002) ArticleTitleHuman chromosome 21 gene expression atlas in the mouse Nature 420 IssueID6915 582–586 Occurrence Handle10.1038/nature01178 Occurrence Handle1:CAS:528:DC%2BD38Xpt1Whs7o%3D Occurrence Handle12466854
L Schibler D Vaiman A Oustry C Giraud-Delville EP Cribiu (1998) ArticleTitleComparative gene mapping: A fine-scale survey of chromosome rearrangements between ruminants and humans Genome Res 8 IssueID9 901–915 Occurrence Handle1:CAS:528:DyaK1cXmsVWgt7w%3D Occurrence Handle9750190
EJ Smith L Shi L Prevost P Drummond S Ramlal G Smith K Pierce J Foster (2001) ArticleTitleExpressed sequence tags for the chicken genome from a normalized, ten-day-oldwhite leghorn whole embryo cDNA library Poult ScL 80 IssueID9 1263–1272 Occurrence Handle1:CAS:528:DC%2BD3MXnt12ntb8%3D
TP Smith SC Fahrenkrug GA Rohrer FA Simmen CE Rexroad JW Keele (2001a) ArticleTitleMapping of expressed sequence tags from a porcine early embryonic cDNA library Anim Genet 32 IssueID2 66–72 Occurrence Handle10.1046/j.1365-2052.2001.00739.x Occurrence Handle1:CAS:528:DC%2BD3MXltFajs7s%3D
TP Smith WM Grosse BA Freking AJ Roberts RT Stone E Casas JE Wray J White J Cho SC Fahrenkrug GL Bennett MP Heaton WW Laegreid GA Rohrer CG Chitko-Mckown G Pertea I Holt S Karamycheva F Liang J Quackenbush JW Keele (2001b) ArticleTitleSequence evaluation of four pooled-tissue normalized bovine cDNA libraries and construction of a gene index for cattle Genome Res. 1 IssueID1(4) 626–630 Occurrence Handle10.1101/gr.170101
PT Spellman GM Rubin (2002) ArticleTitleEvidence for large domains of similarly expressed genes in the Drosophila genome J Biol. 1 IssueID1 5 Occurrence Handle10.1186/1475-4924-1-5 Occurrence Handle12144710
A Takasuga S Hirotsune R Itoh A Jitohzono H Suzuki H Aso Y Sugimoto (2001) ArticleTitleEstablishment of a high throughput EST sequencing system using poly (A) tail-removed cDNA libraries and determination of 36,000 bovine ESTs Nucleic Acids Res 29 IssueID22 E108 Occurrence Handle10.1093/nar/29.22.e108 Occurrence Handle1:STN:280:DC%2BD3Mnmslyruw%3D%3D Occurrence Handle11713328
H Tanabe S Muller M Neusser J Hase ParticleVon E Calcagno M Cremer I Solovei C Cremer T Cremer (2002) ArticleTitleEvolutionary conservation of chromosome territory arrangements in cell nuclei from higher primates Proc Natl Acad Sci USA 99 IssueID7 4424–4429 Occurrence Handle10.1073/pnas.072618599 Occurrence Handle1:CAS:528:DC%2BD38XivFShtLs%3D Occurrence Handle11930003
JW Thomas AB Prasad TJ Summers SQ Lee-Lin VV Maduro JR Idol JF Ryan PJ Thomas JC McDowell ED Green (2002) ArticleTitleParallel construction of orthologous sequence-ready clone contig maps in multiple species Genome Res 12 IssueID8 1277–1285 Occurrence Handle10.1101/gr.283202 Occurrence Handle1:CAS:528:DC%2BD38Xmtl2ruro%3D Occurrence Handle12176935
D Vaiman L Schibler A Oustry-Vaiman E Pailhoux T Goldammer M Stevanovic JP Furet M Schwerin C Cotinot M Fellous EP Cribiu (1999) ArticleTitleHigh-resolution human/goat comparative map of the goat polled/intersex syndrome (PIS): The human homologue is contained in a human YAC from HSA3q23 Genomics 15 IssueID56(1) 31–39 Occurrence Handle10.1006/geno.1998.5691
JC Venter MD Adams EW Myers PW Li RJ Mural GG Sutton HO Smith M Yandell CA Evans RA Holt et al. (2001) ArticleTitleThe sequence of the human genome Science 291 IssueID5507 1304–1351 Occurrence Handle10.1126/science.1058040 Occurrence Handle1:CAS:528:DC%2BD3MXhtlSgsbo%3D Occurrence Handle11181995
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G.M. Cosseddu was funded by EU Marie-Curie fellowship.
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Cosseddu, G.M., Perez-Enciso, M., Fellous, M. et al. Interspecific Chromosome-Wide Transcription Profiles Reveal the Existence of Mammalian-Specific and Species-Specific Chromosome Domains. J Mol Evol 59, 317–328 (2004). https://doi.org/10.1007/s00239-004-2627-6
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DOI: https://doi.org/10.1007/s00239-004-2627-6