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Evidence for Hox Gene Duplication in Rainbow Trout (Oncorhynchus mykiss): A Tetraploid Model Species

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Abstract

We examined the genomic organization of Hox genes in rainbow trout (Oncorhynchus mykiss), a tetraploid teleost derivative species, in order to test models of presumptive genomic duplications during vertebrate evolution. Thirteen putative clusters were localized in the current rainbow trout genetic map; however, analysis of the sequence data suggests the presence of at least 14 Hox clusters. Many duplicated genes appear to have been retained in the genome and share a high percentage of amino acid similarity with one another. We characterized two Hox genes located within the HoxCb cluster that may have been lost independently in other teleost species studied to date. Finally, we identified conserved syntenic blocks between salmonids and human, and provide data supporting two new linkage group homeologies (i.e., RT-3/16, RT-12/29) and three previously described homeologies (RT-2/9, RT-17/22, and RT-27/31) in rainbow trout.

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Acknowledgments

This research was supported by AquaNet, Canada’s Network of Centers of Excellence in aquaculture, and the Natural Sciences and Engineering Research Council of Canada (NSERC). We also wish to thank Dr. Teresa Crease and the JME reviewers for their constructive comments on the manuscript and Xia Yue and Karim Gharbi for their laboratory assistance and technical advice.

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Authors and Affiliations

  1. Department of Integrative Biology, University of Guelph, Guelph, Ontario, Canada, N1G 2W1

    Hooman K. Moghadam, Moira M. Ferguson & Roy G. Danzmann

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  1. Hooman K. Moghadam
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  2. Moira M. Ferguson
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  3. Roy G. Danzmann
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Correspondence to Roy G. Danzmann.

Additional information

The sequence data for this study have been submitted to GenBank under the following accession numbers: AY567792, AY567793, AY567794, AY567795, AY567796, AY567797, AY567798, AY567799, AY567800, AY567801, AY567802, AY567803, AY567804, AY567805, AY567806, AY567807, AY567808, AY567809, AY567810, AY567812, AY567813, AY567814, AY567815, AY567816, and AY567817.

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Moghadam, H.K., Ferguson, M.M. & Danzmann, R.G. Evidence for Hox Gene Duplication in Rainbow Trout (Oncorhynchus mykiss): A Tetraploid Model Species. J Mol Evol 61, 804–818 (2005). https://doi.org/10.1007/s00239-004-0230-5

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