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Protective Effect of Sundakai (Solanum torvum) Seed Protein (SP) Against Oxidative Membrane Damage in Human Erythrocytes

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Abstract

Lipid peroxidation by ROS at the membrane level disturbs the inherit integrity of components activating subsequent alterations in the function. In this study, the protective effect of purified Sundakai (Solanum torvum) seed protein (SP) was tested against oxidative membrane damage in erythrocyte membrane. SP prevented oxidative RBC lysis induced by pro-oxidants; Fe:As (2:20 μmol), periodate (0.4 mM), and t-BOOH (1 mM) up to 86, 81, and 86 %, respectively. Further, SP prevented the Fe:As-induced K+ leakage up to the tune of 95 %. The inhibition offered by SP on K+ leakage was comparable to inhibition offered by quinine sulfate, a known K+ channel blocker. SP dose dependently restored Na+K+ ATPase and Ca2+Mg2+ ATPase activities in erythrocyte membrane. The restoration of ATPase activity by SP was two times more than standard antioxidants BHA and α-tocopherol. Besides, SP at 1.6 μmol restored the membrane proteins over Fe:As induction when analyzed by SDS-PAGE, which was comparable to protection offered by BHA. In conclusion, SP is an effective antioxidant in preventing oxidative membrane damage and associated functions mediated by ROS. As SP is non-toxic, it can be used as an effective bioprotective antioxidant agent to cellular components.

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Abbreviations

BHA:

Butylated hydroxyanisole

Fe:As:

Ferrous sulfate:ascorbic acid

t-BOOH:

Tertiary butyl hydroperoxide

TBA:

Thiobarbituric acid

TBARs:

Thiobarbituric acid reactive substances

MDA:

Malondialdehyde

°C:

Degree Celsius

H:

Hour(s)

Mg:

Milligram

Μg:

Microgram

μM:

Micromolar

nm:

Nanometer

%:

Percentage

SD:

Standard deviation

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Acknowledgments

The authors acknowledge the Adichunchanagiri Shikshana Trust and The principal, Adichunchanagiri Institute of Medical Sciences (AIMS), B.G.Nagar for providing facilities to carry out this work.

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Authors and Affiliations

  1. Aurigene Discovery Technologies, Bangalore, Karnataka, India

    M. Sivapriya

  2. Adichunchanagiri Biotechnology and Cancer Research Institute, Mandya, Karnataka, India

    S. S. Thammanna Gowda & Leela Srinivas

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  1. M. Sivapriya
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  2. S. S. Thammanna Gowda
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  3. Leela Srinivas
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Correspondence to Leela Srinivas.

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Sivapriya, M., Gowda, S.S.T. & Srinivas, L. Protective Effect of Sundakai (Solanum torvum) Seed Protein (SP) Against Oxidative Membrane Damage in Human Erythrocytes. J Membrane Biol 248, 1137–1144 (2015). https://doi.org/10.1007/s00232-015-9831-8

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  • DOI: https://doi.org/10.1007/s00232-015-9831-8

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