Skip to main content

Advertisement

SpringerLink
Log in

A Comparative Study on the Effects of Millisecond- and Microsecond-Pulsed Electric Field Treatments on the Permeabilization and Extraction of Pigments from Chlorella vulgaris

  • Published:
The Journal of Membrane Biology Aims and scope Submit manuscript

Abstract

The interdependencies of the two main processing parameters affecting “electroporation” (electric field strength and pulse duration) while using pulse duration in the range of milliseconds and microseconds on the permeabilization, inactivation, and extraction of pigments from Chlorella vulgaris was compared. While irreversible “electroporation” was observed above 4 kV/cm in the millisecond range, electric field strengths of ≥10 kV/cm were required in the microseconds range. However, to cause the electroporation of most of the 90 % of the population of C. vulgaris in the millisecond (5 kV/cm, 20 pulses) or microsecond (15 kV/cm, 25 pulses) range, the specific energy that was delivered was lower for microsecond treatments (16.87 kJ/L) than in millisecond treatments (150 kJ/L). In terms of the specific energy required to cause microalgae inactivation, treatments in the microsecond range also resulted in greater energy efficiency. The comparison of extraction yields in the range of milliseconds (5 kV, 20 ms) and microseconds (20, 25 pulses) under the conditions in which the maximum extraction was observed revealed that the improvement in the carotenoid extraction was similar and chlorophyll a and b extraction was slightly higher for treatments in the microsecond range. The specific energy that was required for the treatment in the millisecond range (150 kJ/L) was much higher than those required in the microsecond range (30 kJ/L). The comparison of the efficacy of both types of pulses on the extraction enhancement just after the treatment and after a post-pulse incubation period seemed to indicate that PEF in the millisecond range created irreversible alterations while, in the microsecond range, the defects were a dynamic structure along the post-pulse time that caused a subsequent increment in the extraction yield.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

References

  • Barba FJ, Nabil G, Vorobiev E (2015) New approaches for the use of non-conventional cell disruption technologies to extract potential food additives and nutraceuticals from microalgae. Food Eng Rev 7:45–62

    Article  CAS  Google Scholar 

  • Coustets M, Al-Karablieh N, Thomsen C, Teissie J (2013) Flow process for electroextraction of total proteins from microalgae. J Membr Biol 246:751–760

    Article  CAS  PubMed  Google Scholar 

  • Coustets M, Ganeva V, Galutzov B, Teissie J (2015) Millisecond duration pulses for flow-through electroinduced protein extraction from E. coli and associated eradication. Bioelectrochem 103:82–91

    Article  CAS  Google Scholar 

  • De Vito F, Ferrari G, Lebovka NI, Shynkaryk NV, Vorobiev E (2008) Pulse duration and efficiency of soft cellular tissue disintegration by pulsed electric fields. Food Bioprocess Technol 1:307–313

    Article  Google Scholar 

  • Eing C, Goettel M, Straessner M, Gusbeth C, Frey W (2013) Pulsed electric field treatment of microalgae—benefits for microalgae biomass processing. IEEE Trans Plasma Sci 41:2901–2907

    Article  CAS  Google Scholar 

  • Flisar K, Meglic SH, Morelj J, Golob J, Miklavcic D (2014) Testing a prototype pulse generator for a continuous flow system and its use for E. coli inactivation and microalgae lipid extraction. Bioelectrochem 100:44-51

    Article  CAS  Google Scholar 

  • Frey W, Gusbeth C, Schwartz T (2013) Inactivation of Pseudomonas putida by pulsed electric field treatment: a study on the correlation of treatment parameters and inactivation efficiency in the short-pulse range. J Membr Biol 246:769–781

    Article  CAS  PubMed  Google Scholar 

  • Ganeva V, Galutzov B, Eynard N, Teissié J (2001) Electroinduced extraction of β-galactosidase from Kluyveromyces lactis. Appl Microbiol Biotechnol 56:441–413

    Article  Google Scholar 

  • Garcia D, Gomez N, Manas P, Raso J, Pagan R (2007) Pulsed electric fields cause bacterial envelopes permeabilization depending on the treatment intensity, the treatment medium pH and the microorganism investigated. Int J Food Microbiol 113:219–227

    Article  CAS  PubMed  Google Scholar 

  • Goettel M, Eing C, Gusbeth C, Straessner R, Frey W (2013) Pulsed electric field assisted extraction of intracellular valuables from microalgae. Algal Res 2:401–408

    Article  Google Scholar 

  • Gouveia L, Veloso V, Reis A, Fernandes H, Novais J, Empis J (1996) Evolution of pigment composition in Chlorella vulgaris. Bioresour Technol 57:157–163

    Article  CAS  Google Scholar 

  • Grimi N, Dubois A, Marchal L, Jubeau S, Lebovka NI, Vorobiev E (2013) Selective extraction from microalgae Nannochloropsis sp. using different methods of cell disruption. Bioresour Technol 153:254–259

    Article  PubMed  Google Scholar 

  • Ivorra A (2010) Tissue electroporation as a bioelectric phenomenon: basic concepts. In: Rubinsky B (ed) Irreversible electroporation. Springer, Heidelberg, pp 23–61

    Chapter  Google Scholar 

  • Kotnik T, Kramar P, Pucihar G, Miklavcic D, Tarek M (2012) Cell membrane electroporation—part 1: the phenomenon. Electr Insul Mag 28:14–23

    Article  Google Scholar 

  • Lichtenthaler H (1987) Chlorophyll and carotenoids: pigments of photosynthetic biomembranes. Methods Enzymol 148:350–382

    Article  CAS  Google Scholar 

  • Luengo E, Condon-Abanto S, Alvarez I, Raso J (2014) Effect of pulsed electric field treatments on permeabilization and extraction of pigments from Chlorella vulgaris. J Membr Biol 247:1269–1277

    Article  CAS  PubMed  Google Scholar 

  • Mahnic-Kalamiza S, Vorobiev E, Miklavcic D (2014) Electroporation in food processing and biorefinery. J Membr Biol 247:1279–1304

    Article  CAS  PubMed  Google Scholar 

  • Ohshima T, Okuyama K, Sato M (2002) Effect of culture temperature on high-voltage pulse sterilization of Escherichia coli. J Electrostat 55:227–235

    Article  Google Scholar 

  • Parniakov O, Barba FJ, Grimi N, Marchal L, Jubeau S, Lebovka N, Vorobiev E (2015) Pulsed electric field assisted extraction of nutritionally valuable compounds from microalgae Nannochloropsis spp. using the binary mixture of organic solvents and water. Innov Food Sci Emerg Technol 27:79–85

    Article  CAS  Google Scholar 

  • Qin S, Timoshkin IV, Maclean M, Wilson MP, MacGregor SJ, Given MJ, Anderson JG, Wang T (2014) Pulsed electric field treatment of microalgae: inactivation tendencies and energy consumption. IEEE Trans Plasma Sci 42:3191–3196

    Article  Google Scholar 

  • Raso J, Alvarez I, Condón S, Sala Trepat FJ (2000) Predicting inactivation of Salmonella senftenberg by pulsed electric fields. Innov Food Sci Emerg Technol 1:21–29

    Article  Google Scholar 

  • Rodrigo D, Ruíz P, Barbosa-Cánovas GV, Martínez A, Rodrigo M (2003) Kinetic model for the inactivation of Lactobacillus plantarum by pulsed electric fields. Int J Food Microbiol 81:223–229

    Article  CAS  PubMed  Google Scholar 

  • Rols MP, Delteil C, Golzio M, Teissie J (1998) Control by ATP and ADP of voltage-induced mammalian-cell-membrane permeabilization, gene transfer and resulting expression. Eur J Biochem 254:382–388

    Article  CAS  PubMed  Google Scholar 

  • Sheng J, Vannela R, Rittmann BE (2011) Evaluation of cell disruption effects of pulsed-electric-field treatment of Synechocystis PCC 6803. Environ Sci Technol 45:3795–3802

    Article  CAS  PubMed  Google Scholar 

  • Teissie J, Golzio M, Rols MP (2005) Mechanisms of cell membrane electropermeabilization: a minireview of our present (lack of ?) knowledge. Biochim Biophys Acta 1724:270–280

    Article  CAS  PubMed  Google Scholar 

  • Toepfl S, Heinz V, Knorr D (2006a) Applications of pulsed electric fields technology for the food industry. In: Raso J, Heinz V (eds) Pulsed electric fields technology for the food industry. Springer, New York, pp 197–221

    Chapter  Google Scholar 

  • Toepfl S, Mathys A, Heinz V, Knorr D (2006b) Review: potential of high hydrostatic pressure and pulsed electric fields for energy efficient and environmentally friendly food processing. Food Rev Int 22:405–423

    Article  CAS  Google Scholar 

  • Weaver JC, Smith KC, Esser AT, Son RS, Gowrishankar TR (2012) A brief overview of electroporation pulse strength-duration space: a region where additional intracellular effects are expected. Bioelectrochemistry 87:236–243

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Zbinden MDA, Sturm BSM, Nord RD, Carey WJ, Moore D, Shinogle H, Stagg-Williams SM (2013) Pulsed electric field (PEF) as an intensification pretreatment for greener solvent lipid extraction from microalgae. Biotechnol Bioeng 110:1605–1615

    Article  PubMed  Google Scholar 

Download references

Acknowledgments

This research was supported by the Government of Aragón (Grupo de Investigación Consolidado A20), and the European Social Fund. E. Luengo gratefully acknowledges the financial support for her doctoral studies from the Department of Science, Technology and University of the Aragon Government. This manuscript is a result of the networking efforts of the European Cooperation in Science and Technology (COST) Action TD1104.

Author information

Authors and Affiliations

  1. Tecnología de los Alimentos, Facultad de Veterinaria, Universidad de Zaragoza, C/Miguel Servet, 177, 50013, Saragossa, Spain

    Elisa Luengo, Juan Manuel Martínez, Ignacio Álvarez & Javier Raso

  2. CNRS, IPBS (Institut de Pharmacologie et de Biologie Structurale), BP 64182, 205 route de Narbonne, 31077, Toulouse, France

    Mathilde Coustets, Justin Teissié & Marie-Pierre Rols

  3. IPBS (Institut de Pharmacologie et de Biologie Structurale), Université de Toulouse, UPS (Université Paul Sabatier), BP 64182, 31077, Toulouse, France

    Mathilde Coustets, Justin Teissié & Marie-Pierre Rols

Authors
  1. Elisa Luengo
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Juan Manuel Martínez
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Mathilde Coustets
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Ignacio Álvarez
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Justin Teissié
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Marie-Pierre Rols
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Javier Raso
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Javier Raso.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Luengo, E., Martínez, J.M., Coustets, M. et al. A Comparative Study on the Effects of Millisecond- and Microsecond-Pulsed Electric Field Treatments on the Permeabilization and Extraction of Pigments from Chlorella vulgaris . J Membrane Biol 248, 883–891 (2015). https://doi.org/10.1007/s00232-015-9796-7

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00232-015-9796-7

Keywords

Search

Navigation