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Effect of Biotin and Galactose Functionalized Gelatin Nanofiber Membrane on HEp-2 Cell Attachment and Cytotoxicity

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Abstract

In the present study, we prepared a gelatin nanofiber matrix using an electrospinning technique and cross-linked the nanofibers with 10 % glutaraldehyde vapors. The insoluble nanofibers were functionalized with bioactive molecules like biotin (1 %) and galactose (1 %) by adsorption and coelectrospinning. Surface morphology and fiber dimension were analyzed using atomic force microscopy. The amounts of biotin and galactose bound to the nanofibers before and after adsorption were quantified using high-performance liquid chromatography. Human larynx carcinoma (HEp-2) cell attachment, morphology and cytotoxic characteristics were studied using crystal violet staining and the MTT assay. Cell attachment and viability were highest in biotin- and galactose-embedded nanofibers compared to native nanofibers. Cytotoxicity was less with biotin- and galactose-embedded and adsorbed nanofibers compared to control nanofibers. Hence, we suggest that these biocompatible, nontoxic, biodegradable, functionalized nanofibers could be a potential candidate for application in tissue engineering and scaffold preparation.

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Acknowledgments

The authors acknowledge Dr. V. Ramamurthy, professor and head, Department of Biotechnology, for his help in allowing us to use the lab facility. The authors also acknowledge Mr. K. Karthikeyan for help in AFM analysis.

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Authors and Affiliations

  1. Nanobiotechnology Laboratory, PSG Institute of Advanced Studies, Peelamedu, Coimbatore, 641004, India

    R. Selvakumar, S. Nazar Mohamed Mohaideen & S. Aravindh

  2. Department of Biotechnology, PSG College of Technology, Peelamedu, Coimbatore, 641004, India

    S. Nazar Mohamed Mohaideen, C. Sabarinath & M. Ananthasubramanian

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  1. R. Selvakumar
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  2. S. Nazar Mohamed Mohaideen
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  3. S. Aravindh
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  5. M. Ananthasubramanian
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Correspondence to R. Selvakumar.

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Selvakumar, R., Mohaideen, S.N.M., Aravindh, S. et al. Effect of Biotin and Galactose Functionalized Gelatin Nanofiber Membrane on HEp-2 Cell Attachment and Cytotoxicity. J Membrane Biol 247, 35–43 (2014). https://doi.org/10.1007/s00232-013-9608-x

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  • DOI: https://doi.org/10.1007/s00232-013-9608-x

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