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Serum Paraoxonase and Arylesterase Activities and Oxidative Stress Levels in Patients with SSRI Intoxication

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Abstract

Oxidative stress is a critical route of damage in various psychological stress-induced disorders, such as depression. Paraoxonase-1 (PON1) plays an important role as an endogenous free-radical scavenging molecule. The aim of this study was to evaluate the influence of serum PON1 activity and oxidative stress in patients with selective serotonin reuptake inhibitor (SSRI) intoxication. A total of 11 patients with SSRI intoxication and 20 healthy controls were enrolled. The serum total antioxidant capacity (TAC) and malondialdehyde (MDA) levels, as well as the paraoxonase and arylesterase activities, were measured spectrophotometrically. The serum TAC levels and the paraoxonase and arylesterase activities were significantly lower (for all, p < 0.001), whereas the serum MDA levels were significantly higher in the patients with SSRI intoxication than in the controls (p < 0.001). These results indicated that decreased PON1 activity and increased oxidative stress represent alternative mechanisms in SSRI toxicity. More studies are needed to elucidate the role of PON1 activity in the etiology of SSRI intoxication.

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References

  • Abdel-Salam OM, Youness ER, Khadrawy YA, Sleem AA (2013) Brain and liver oxidative stress after sertraline and haloperidol treatment in mice. J Basic Clin Physiol Pharmacol 24(2):115–123

    CAS  PubMed  Google Scholar 

  • Aitken RJ, Roman SD (2008) Antioxidant systems and oxidative stress in the testes. Oxid Med Cell Longev 1:15–24

    Article  PubMed Central  PubMed  Google Scholar 

  • Alagoz G, Durukan P, Yıldız M, Bayar MK, Ilhan N, Cevik Y, Seckin D (2007) Investigation of relationship among serum malondialdehyde, paraoxonase and liver function tests in acute poisoning patients. Firat Med J 12(3):184–189

    CAS  Google Scholar 

  • Ali EM, Shehata HH, Ali-Labib R et al (2009) Oxidant and antioxidant of arylesterase and paraoxonase as biomarkers in patients with hepatitis C virus. Clin Biochem 42:1394–1400

    Article  CAS  PubMed  Google Scholar 

  • Ates O, Azizi S, Alp HH et al (2009) Decreased serum paraoxonase 1 activity and increased serum homocysteine and malondialdehyde levels in age-related macular degeneration. Tohoku J Exp Med 217:17–22

    Article  CAS  PubMed  Google Scholar 

  • Aviram M, Rosenblat M, Billecke S et al (1999) Human serum paraoxonase (PON 1) is inactivated by oxidized low density lipoprotein and preserved by antioxidants. Free Radic Biol Med. 26:892–904

    Article  CAS  PubMed  Google Scholar 

  • Aviram M, Hardak E, Vaya J et al (2000) Human serum paraoxonases (PON1) Q and R selectively decrease lipid peroxides in coronary and carotid atherosclerotic lesions: PON1 esterase and peroxidase-like activities. Circulation 101:2510–2517

    Article  CAS  PubMed  Google Scholar 

  • Bilici M, Efe H, Koroglu MA, Uydu HA, Bekaroglu M, Deger O (2001) Antioxidative enzyme activities and lipid peroxidation in major depression: alterations by antidepressant treatments. J Affect Disord 64:43–51

    Article  CAS  PubMed  Google Scholar 

  • Blatter MC, James RW, Messmer S et al (1993) Identification of a distinct human high-density lipoprotein subspecies defined by a lipoprotein-associated protein, K-45. Identity of K-45 with paraoxonase. Eur J Biochem 211:871–879

    Article  CAS  PubMed  Google Scholar 

  • Chandra M, Chandra N, Agrawal R et al (1994) The free radical system in ischemic heart disease. Int J Cardiol 43:121–125

    Article  CAS  PubMed  Google Scholar 

  • Delattre J, Beaudeux JL, Bonnefont-Rousselot D (2005) Radicaux libres et stress oxydant. Aspects biologiques et pathologiques. Éditions Médicales internationales, Paris, pp 1–492

    Google Scholar 

  • Ditzen C, Jastorff AM, Keßler MS, Bunck M, Teplytska L, Erhardt A, Krömer SA, Varadarajulu J, Targosz BS, Sayan-Ayata EF, Holsboer F, Landgraf R, Turck CW (2006) Protein biomarkers in a mouse model of extremes in trait anxiety. Mol Cell Proteomics 5:1914–1920

    Article  CAS  PubMed  Google Scholar 

  • Eckerson HW, Wyte MC, La Du BN (1983) The human serum paraoxonase/arylesterase polymorphism. Am J Hum Genet 35:1126–1138

    PubMed Central  CAS  PubMed  Google Scholar 

  • Erel O (2004) A novel automated direct measurement method for total antioxidant capacity using a new generation more stable ABTS radical cation. Clin Biochem 37:277–285

    Article  CAS  PubMed  Google Scholar 

  • Ferré N, Camps J, Prats E, Vilella E, Paul A, Figuera L et al (2002) Serum paraoxonase activity: a new additional test for the improved evaluation of chronic liver damage. Clin Chem 48:261–268

    PubMed  Google Scholar 

  • Fujii J, Iuchi Y, Matsuki S, Ishii T (2003) Cooperative function of antioxidant and redox systems against oxidative stress in male reproductive tissues. Asian J Androl 5:231–242

    CAS  PubMed  Google Scholar 

  • Gury C, Cousin F (1999) Pharmacokinetics of SSRI antidepressants: half-life and clinical applicability. Encephale 25:470–476

    CAS  PubMed  Google Scholar 

  • Gutteridge JM (1995) Lipid peroxidation and antioxidants as biomarkers of tissue damage. Clin Chem 4:1819–1828

    Google Scholar 

  • Haagen L, Brock A (1992) A new automated method for phenotyping arylesterase (E.C. 3.1.1.2) based upon inhibition of enzymatic hydrolysis of 4-nitrophenyl acetate by phenyl acetate. Eur J Clin Chem Clin Biochem 30:391–395

    CAS  PubMed  Google Scholar 

  • Kabaroglu C, Mutaf I, Boydak B et al (2004) Association between serum paraoxonase activity and oxidative stress in acute coronary syndromes. Acta Cardiol 59:606–611

    Article  PubMed  Google Scholar 

  • Kilic SS, Aydin S, Kilic N, Erman F, Aydin S, Celik I (2005) Serum arylesterase and paraoxonase activity in patients with chronic hepatitis. World J Gastroenterol 11:7351–7354

    Article  CAS  PubMed  Google Scholar 

  • Kirino E (2012) Escitalopram for the management of major depressive disorder: a review of its efficacy, safety, and patient acceptability. Patient Prefer Adherence 6:853–861

    Article  PubMed Central  PubMed  Google Scholar 

  • Kotur-Stevuljevic J, Spasic S, Jelic-Ivanovic Z et al (2008) PON1 status is influenced by oxidative stress and inflammation in coronary heart disease patients. Clin Biochem 41:1067–1073

    Article  CAS  PubMed  Google Scholar 

  • Krömer SA, Keßler MS, Milfay D, Birg IN, Bunck M, Czibere L, Panhuysen M, Pütz B, Deussing JM, Holsboer F, Landgraf R, Turck CW (2005) Identification of glyoxalase-I as a protein marker in a mouse model of extremes in trait anxiety. J Neurosci 25:4375–4384

    Article  PubMed  Google Scholar 

  • Leviev I, Negro F, James RW (1997) Two alleles of the human paraoxonase gene produce different amounts of mRNA. Arterioscler Thromb Vasc Biol 17:2935–2939

    Article  CAS  PubMed  Google Scholar 

  • Mackness MI, Mackness B, Durrington PN, Connelly PW, Hegele RA (1996) Paraoxonase: biochemistry, genetics and relationship to plasma lipoproteins. Curr Opin Lipidol 7:69–76

    Article  CAS  PubMed  Google Scholar 

  • Mackness M, Durrington P, Mackness B (2004) Paraoxonase 1 activity, concentration and genotype in cardiovascular disease. Curr Opin Lipidol 15:399–404

    Article  CAS  PubMed  Google Scholar 

  • Rozenberg O, Rosenblat M, Coleman R et al (2003) Paraoxonase (PON1) deficiency is associated with increased macrophage oxidative stress: studies in PON1-knock out mice. Free Radic Biol Med 34:774–784

    Article  CAS  PubMed  Google Scholar 

  • Saadaoui MH, Hellara I, Neffati F, Mechri A, Douki W, Gaha L, Najjar MF (2012) In vitro effects of three antidepressant drugs on plasma paraoxonase activity. Drug Metabol Drug Interact 27(4):209–215

    Article  CAS  PubMed  Google Scholar 

  • Sams C, Mason HJ (1999) Detoxification of organohosphates by A-esterases in human serum. Hum Exp Toxicol 18:653–658

    Article  CAS  PubMed  Google Scholar 

  • Shamir R, Hartman C, Karry R et al (2005) Paraoxonases (PONs) 1, 2 and 3 are expressed in human and mouse gastrointestinal tract and in Caco-2 cell line: selective secretion of PON1 and PON2. Free Radic Biol Med 39:336–344

    Article  CAS  PubMed  Google Scholar 

  • Torun AN, Kulaksizoglu S, Kulaksizoglu M, Pamuk BO, Isbilen E, Tutuncu NB (2009) Serum total antioxidant status and lipid peroxidation marker malondialdehyde levels in overt and subclinical hypothyroidism. Clin Endocrinol (Oxf) 70:469–474

    Article  Google Scholar 

  • Tuthill A, Slawik H, O’Rahilly S, Finer N (2006) Psychiatric co-morbidities in patients attending specialist obesity services in the UK. QJM 99:317–325

    Article  CAS  PubMed  Google Scholar 

  • Valko M, Leibfritz D, Moncol J, Cronin MTD, Mazur M, Telser J (2007) Free radicals and antioxidants in normal physiological functions and human disease. Int J Biochem Cell B 39:44–84

    Article  CAS  Google Scholar 

  • Wittchen HU, Jacobi F (2005) Size and burden of mental disorders in Europe—a critical review and appraisal of 27 studies. Eur J Neuropsychopharmacol 15:357–376

    Article  CAS  Google Scholar 

  • Yildiz A, Gür M, Yilmaz R, Demirbağ R, Celik H, Aslan M, Koçyiğit A (2008) Lymphocyte DNA damage and total antioxidant status in patients with white-coat hypertension and sustained hypertension. Turk Soc Cardiol 36:231–238

    Google Scholar 

  • Yoshioka T, Kawada K, Shimada T et al (1979) Lipid peroxidation in maternal and cord blood and protective mechanisms against activated oxygen toxicity in the blood. Am J Obstet Gynecol 135:372–376

    CAS  PubMed  Google Scholar 

  • Zafarullah M, Li WQ, Sylvester J, Ahmad M (2003) Molecular mechanisms of N-acetylcysteine actions. Cell Mol Life Sci 60:6–20

    Article  CAS  PubMed  Google Scholar 

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The authors do not report any conflicts of interest regarding this work.

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Authors and Affiliations

  1. Department of Emergency Medicine, Ondokuz Mayıs University, Samsun, Turkey

    Celal Katı & Latif Duran

  2. Department of Emergency Medicine, Medical Faculty, Yuzuncu Yıl University, Van, Turkey

    Sevdegul Karadas & Hayriye Gonullu

  3. Department of Internal Medicine, Medical Faculty, Yuzuncu Yıl University, 65000, Van, Turkey

    Mehmet Aslan

  4. Department of Chemistry, Faculty of Science and Art, Yuzuncu Yıl University, Van, Turkey

    Halit Demir

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  1. Celal Katı
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  2. Sevdegul Karadas
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  3. Mehmet Aslan
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  4. Hayriye Gonullu
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  5. Latif Duran
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Correspondence to Mehmet Aslan.

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Katı, C., Karadas, S., Aslan, M. et al. Serum Paraoxonase and Arylesterase Activities and Oxidative Stress Levels in Patients with SSRI Intoxication. J Membrane Biol 247, 17–21 (2014). https://doi.org/10.1007/s00232-013-9606-z

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  • DOI: https://doi.org/10.1007/s00232-013-9606-z

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