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Sorption of Copper and Zinc to the Plasma Membrane of Wheat Root

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Abstract

Sorption of Cu2+ and Zn2+ to the plasma membrane (PM) of wheat root (Triticum aestivum L␣cv. Scout 66) vesicles was measured at different pH values and in the presence of organic acids and other metals. The results were analyzed using a Gouy-Chapman-Stem model for competitive sorption (binding and electrostatic attraction) to a negative binding site. The binding constants for the two investigated cations as evaluated from the sorption experiments were 5 M−1 for Zn2+ and 400 M−1 for Cu2+. Thus, the sorption affinity of Cu2+ to the PM is considerably larger than that of Ca2+, Mg2+ or Zn2+. The greater binding affinity of Cu2+ was confirmed by experiments in which competition with La3+ for sorption sites was followed. The amount of sorbed Cu2+ decreased with increasing K+, Ca2+, or La3+ concentrations, suggesting that all these cations competed with Cu2+ for sorption at the PM binding sites, albeit with considerable differences among these cations in effectiveness as competitors with Cu2+. The sorption of Cu2+ and Zn2+ to the PM decreased in the presence of citric acid or malic acid. Citric acid (as well as pH) affected the sorption of Cu2+ or Zn2+ to PM more strongly then did malic acid.

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References

  • A.H. Berglund M.F. Quartacci L. Calucci I.F. Navari C. Pinzino C. Liljenberg (2002) ArticleTitleAlterations of wheat root plasma membrane lipid composition induced by copper stress result in changed physicochemical properties of plasma membrane lipid vesicles Biochim. Biophys. Acta 1564 466–472

    Google Scholar 

  • M.M. Bradford (1979) ArticleTitleA rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding Anal. Biochem. 72 248–254

    Google Scholar 

  • D.K. Brauer U. Yermiyahu G. Rytwo T.B. Kinraide (2000) ArticleTitleCharacteristics of the quenching of 9-aminoacridine fluorescence by liposomes made from plant lipids J. Membrane Biol. 178 43–48

    Google Scholar 

  • W.S. Chow J. Barber (1980) ArticleTitle9-aminoacridine fluorescence changes as a measure of surface charge density of thylakoid membrane Biochim. Biophys. Acta 589 346–352

    Google Scholar 

  • P.G.C. Campbell (1995) Interactions between trace metals and aquatic organisms: A critique of the free-ion activity model A. Tessier D.R. Turner (Eds) Metal Speciation, Bioavailability in Aquatic Systems John Wiley & Sons New York 45–102

    Google Scholar 

  • D.T. Clarkson J.B. Hanson (1980) ArticleTitleThe mineral nutrition of higher plant Annu. Rev. Plant Physiol. 31 239–298

    Google Scholar 

  • S.D. Edds L.V. Kochian (1997) ArticleTitleToxicity of zinc and copper to Brassica species: implications for phytoremediation J. Environ. Qual. 26 776–781

    Google Scholar 

  • R. Gabrielli T. Pandolfini (1984) ArticleTitleEffect of Mg2+ and Ca2+ on the response to nickel toxicity in a serpentine endemic and nickel-accumulating species Physiol. Plant. 62 540–544

    Google Scholar 

  • U. Galli H. Schuepp C. Brunold (1996) ArticleTitleThiols in cadmium and copper treated maize (Zea mays L.) Planta 198 139–143

    Google Scholar 

  • D. Jowett (1964) ArticleTitlePopulation studies on lead-tolerant Agrostis tenuis Evolution 18 70–81

    Google Scholar 

  • T.B. Kinraide (1998) ArticleTitleThree mechanisms for the calcium alleviation of mineral toxicities Plant Physiol. 118 513–520

    Google Scholar 

  • T.B. Kinraide (2003) ArticleTitleThe controlling influence of cell-surface electrical potential on the uptake and toxicity of selenate (SeO 2−4 ) Physiol. Plantarum 117 64–71

    Google Scholar 

  • T.B. Kinraide (2004) ArticleTitlePossible influence of cell walls upon ion concentrations at plasma membrane surfaces. Toward a comprehensive view of cell-surface electrical effects upon ion uptake, intoxication, and amelioration Plant Physiol. 136 3804–3813

    Google Scholar 

  • T.B. Kinraide J.F. Pedler D.R. Parker (2004) ArticleTitleRelative effectiveness of calcium and magnesium in the alleviation of rhizotoxicity in wheat induced by copper, zinc, aluminum, sodium, and low pH Plant Soil 259 201–208

    Google Scholar 

  • T.B. Kinraide U. Yermiyahu G. Rytwo (1998) ArticleTitleComputation of surface electrical potentials of plant cell membranes. Correspondence to published zeta potentials from diverse plant sources Plant Physiol. 118 505–512

    Google Scholar 

  • C. Larsson S. Widell M. Sommarin (1988) ArticleTitleInside-out plant plasma membrane vesicles of high purity obtained by aqueous two-phase partitioning Fed. Eur. Biochem. Soc. Letters 2 289–292

    Google Scholar 

  • W.L. Lindsay (1979) Chemical Equilibria in Soil John Wiley & Sons New York

    Google Scholar 

  • I.M. Møller T. Lundborg A. Bérczi (1984) ArticleTitleThe negative surface charge density of plasmalemma vesicles from wheat and oat roots Fed. Eur. Biochem. Soc. Letters 167 181–185

    Google Scholar 

  • A.S. Murphy W.R. Eisinger J.E. Shaff L.V.L. Kochian Taiz (1999) ArticleTitleEarly copper-induced leakage of K+ from Arabidopsis seedling is mediated by ion channels and coupled to citrate efflux Plant Physiol. 121 1375–1382

    Google Scholar 

  • S. Nir (1984) ArticleTitleA model for cation adsorption in closed systems: Application to calcium binding to phospholipid vesicles J. Colloid Interface Sci. 102 313–321

    Google Scholar 

  • I. Obi Y. Ichikawa T. Kakutani M. Senda (1989) ArticleTitleElectrophoresis, zeta potential and surface charges of barley mesophyll protoplasts Plant Cell Physiol. 30 129–135

    Google Scholar 

  • D.R. Parker W.A. Norvell R.L. Chaney (1995) GEOCHEM-PC – a chemical speciation program for IBM and compatible personal computers A.P. Loeppert R.H. Schwab S. Goldberg (Eds) Chemical Equilibrium, Reaction Models Soil Science Society of America Madison, WI 253–269

    Google Scholar 

  • D.R. Parker J.F. Pedler D.N. Thomasom L. Huiying (1998) ArticleTitleAlleviation of copper rhizotoxicity by calcium and magnesium at defined free metal-ion activities Soil Sci. Soc. Am. J. 62 965–972

    Google Scholar 

  • J.F. Pedler T.B. Kinraide D.R. Parker (2004) ArticleTitleZinc rhizotoxicity in wheat and radish is alleviated by micromolar levels of magnesium and potassium in solution culture Plant Soil 259 191–199

    Google Scholar 

  • A.D. Robson J.F. Loneragan (1970) ArticleTitleSensitivity of annual Medicago species to manganese toxicity as affected by calcium and pH Aust. J. Agric. Res. 21 223–232

    Google Scholar 

  • R.R. Ryan E. Delhaize (2001) ArticleTitleFunction and mechanism of organic anion exudation from plant roots Annu. Rev. Plant Physiol. Plant Mol. Biol. 52 527–560

    Google Scholar 

  • I. Shomer A. Novacky S. Pike U. Yermiyahu T.B. Kinraide (2003) ArticleTitleElectrical potentials of plant cell walls in response to the ionic environment Plant Physiol. 133 411–422

    Google Scholar 

  • I.R. Silva T.J. Smyth D.W. Israel C.D. Paper T.W. Rufty (2001) ArticleTitleMagnesium is more efficient than calcium in alleviating aluminum rhizotoxicity in soybean and its ameliorative effect is not explained by the Gouy-Chapman-Stern model Plant Cell Physiol. 42 538–545

    Google Scholar 

  • S.A. Tatulian (1999) Surface electrostatics of biological membranes and ion binding T.S. Sørensen (Eds) Surface Chemistry and Electrochemistry of Membranes Marcel Dekker New York 871–922

    Google Scholar 

  • U. Yermiyahu S. Nir G. Ben-Hayyim U. Kafkafi (1994) ArticleTitleQuantitative competition of calcium with sodium or magnesium for sorption sites on plasma membrane vesicles of melon (Cucumis melo L.) root cells J. Membrane Biol. 138 55–56

    Google Scholar 

  • U. Yermiyahu D.K. Brauer T.B. Kinraide (1997a) ArticleTitleSorption of aluminum to plasma membrane vesicles isolated from roots of Scout 66 and Atlas 66 cultivars of wheat Planet Physiol. 115 1119–1125

    Google Scholar 

  • U. Yermiyahu S. Nir G. Ben-Hayyim U. Kafkafi T.B. Kinraide (1997b) ArticleTitleRoot elongation in saline solution related to calcium␣binding to root cell plasma membranes Plant Soil 191 67–76

    Google Scholar 

  • U. Yermiyahu G. Rytwo D.K. Brauer T.B. Kinraide (1997c) ArticleTitleBinding and electrostatic attraction of lanthanum (La3+) and aluminum (Al3+) to wheat root plasma membranes J. Membrane Biol. 159 239–252

    Google Scholar 

  • Q. Zhang F.A. Smith A.H. Sekimoto R.J. Reid (2001) ArticleTitleEffect of membrane surface charge on nickel uptake by purified mung bean root protoplasts Planta 213 788–793

    Google Scholar 

Download references

Acknowledgments

This research was supported by Research Grant Award No. IS-3120-99R from BARD, The United States - Israel Binational Agricultural Research and Development Fund.

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Authors and Affiliations

  1. Agricultural Research Organization, Gilat Research Center, D.N. Negev 2 85280, Israel

    R. Vulkan, U. Yermiyahu, U. Mingelgrin, G. Rytwo & T.B. Kinraide

  2. Agricultural Research Organization, Bet Dagan, Israel P.O. Box 6 50250, Israel

    U. Mingelgrin

  3. Dept. of Biotechnology & Environmental Sciences, Tel Hai Academic College, Upper Galilee, 12210, Israel

    G. Rytwo

  4. Appalachian Farming Systems Research Center, Agricultural Research Service, United States Department of Agriculture, Beaver, WV, 25813-9423, USA

    T.B. Kinraide

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  1. R. Vulkan
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  2. U. Yermiyahu
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  4. G. Rytwo
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  5. T.B. Kinraide
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Correspondence to U. Yermiyahu.

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Vulkan, R., Yermiyahu, U., Mingelgrin, U. et al. Sorption of Copper and Zinc to the Plasma Membrane of Wheat Root. J Membrane Biol 202, 97–104 (2004). https://doi.org/10.1007/s00232-004-0722-7

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  • DOI: https://doi.org/10.1007/s00232-004-0722-7

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