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Cytochrome P4502E1 (CYP2E1) expression in peripheral blood lymphocytes: evaluation in hepatitis C and diabetes

  • Pharmacokinetics and Disposition
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Abstract

Objective

Cytochrome P4502E1 (CYP2E1) is expressed in human peripheral blood lymphocytes (PBLs), and previous reports have suggested the possibility of using this readily available tissue as a reporter of CYP2E1 status. To further explore the relevance of this approach we assessed CYP2E1 expression in PBLs in two contrasting conditions, chronic hepatitis C and insulin-dependent diabetes (IDD), illustrating an organ and a systemic disease, respectively.

Methods

Total RNA was isolated from extracted PBLs (hepatitis C patients + IDD) and by percutaneous needle biopsy (hepatitis C patients only). Gene expression for CYP2E1 was determined by real-time reverse-transcription polymerase chain reaction. Histological changes in liver tissue were assessed according to Ludwig's criteria.

Results

In patients with chronic hepatitis C a clear relationship was found between CYP2E1 expression in the liver and the progression of hepatic disease (both lobular inflammation and fibrosis indices), and observed variations were consistent with the preferential distribution of CYP2E1 in the lobular zone. No effect of the liver disease was, however, found at the PBL level. A statistically significant increase in mean CYP2E1 expression level was observed in the lymphocytes from poorly controlled IDD subjects compared to controls.

Conclusions

Taken together, our data indicate that the measurement of CYP2E1 expression in PBLs is not useful in liver diseases. However, in a systemic condition (IDD) this measurement can be proposed for monitoring the CYP2E1 induction in a relatively noninvasive manner. This tool should therefore be further validated in clinical field or experimental studies for CYP2E1 phenotyping purposes.

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Acknowledgements

This study was supported by the National Fund for Scientific Research, Belgium, and by the European Commission (Environment and Health, fifth framework program, contract QLRT-1999-01368). Authors thank Dr. D. Maisin for the measurements of HbA1C. The technical assistance of F. Toubeau is also gratefully acknowledged.

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Authors and Affiliations

  1. Industrial Toxicology and Occupational Medicine Unit, Catholic University of Louvain, Clos Chapelle-aux-Champs 30/54, 1200, Brussels, Belgium

    Vincent Haufroid, Danuta Ligocka & Dominique Lison

  2. Department of Endocrinology and Nutrition, St. Luc Hospital, Catholic University of Louvain, Av. Hippocrate 10, 1200, Brussels, Belgium

    Martin Buysschaert

  3. Department of Gastroenterology, St. Luc Hospital, Catholic University of Louvain, Av. Hippocrate 10, 1200, Brussels, Belgium

    Yves Horsmans

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  1. Vincent Haufroid
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  2. Danuta Ligocka
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  3. Martin Buysschaert
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  4. Yves Horsmans
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  5. Dominique Lison
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Correspondence to Vincent Haufroid.

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Haufroid, V., Ligocka, D., Buysschaert, M. et al. Cytochrome P4502E1 (CYP2E1) expression in peripheral blood lymphocytes: evaluation in hepatitis C and diabetes. Eur J Clin Pharmacol 59, 29–33 (2003). https://doi.org/10.1007/s00228-003-0566-6

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  • DOI: https://doi.org/10.1007/s00228-003-0566-6

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