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p53 sequence polymorphisms in late-stage leukemic Mytilus edulis are homologous with M. trossulus p53

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Abstract

Mytilus edulis and M. trossulus are key species in marine intertidal communities that develop a leukemia-like haemic neoplasia at increased incidences at some polluted sites. The tumor-suppressor p53, known to be involved in this disease, was proposed as a biomarker based on sequence polymorphisms in M. trossulus. In this study, p53 mRNA sequence variations in normal and diseased M. edulis were compared with those reported previously for M. trossulus for caged individuals of both species submerged in Burrard Inlet (49°20′16.57″N 123°13′51.37″) in 2007 and 2010. Thirty-seven single nucleotide polymorphisms (SNPs) were detected in M. edulis p53 that formed high linkage disequilibrium blocks associated with late-stage leukemia (p < 0.05). These were found at loci that were more homologous with M. trossulus than with healthy M. edulis p53-predicted haplotypes. Increased susceptibility to this cancer might be due to, in part, p53 alleles containing these SNPs.

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Acknowledgments

Funding was generously provided by the Natural Sciences and Engineering Research Council of Canada Collaborative Research and Development Grant program (CRDPJ 349918) and Metro Vancouver (Head Office: 4330 Kingsway, Burnaby, B.C. V5H 4G8). Dr. Annette Muttray is acknowledged for arranging the collection and shipping of M. edulis from Prince Edward Island.

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  1. Chemical and Biological Engineering, University of British Columbia, 2360 East Mall, Vancouver, BC, V6T 1Z3, Canada

    Ekaterina Vassilenko & Susan A. Baldwin

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  1. Ekaterina Vassilenko
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  2. Susan A. Baldwin
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Correspondence to Susan A. Baldwin.

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Communicated by T. Reusch.

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Vassilenko, E., Baldwin, S.A. p53 sequence polymorphisms in late-stage leukemic Mytilus edulis are homologous with M. trossulus p53. Mar Biol 160, 1751–1760 (2013). https://doi.org/10.1007/s00227-013-2227-3

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  • DOI: https://doi.org/10.1007/s00227-013-2227-3

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