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Changes in metabolic substrates during early development in anchoveta Engraulis ringens (Jenyns 1842) in the Humboldt Current

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Abstract

We assessed the ontogenetic changes in protein content and free amino acids (FAA) in eggs and early larvae of Engraulis ringens (anchoveta) off central Chile on different dates during the spawning season. On all sampling dates, a reduction in embryonic yolk-sac volume, proteins and FAA concentrations occurred during development. Protein electrophoresis (SDS–PAGE) of eggs and larvae showed at least 22 protein bands: 11 were consumed early and not detected after hatching. The proportion of essential FAA (EFAA) was higher than the proportion of non-essential FAA (NEFAA) in early eggs and in 7 day-old larvae (82.5-73% EFAA respectively). During egg development, the FAA pool was dominated by leucine, alanine and lysine, three amino acids contributing 35–44% of the total FAA in eggs. During larval development, histidine was the most abundant FAA. In July, total FAA constituted 13–18% of the egg dry weight. A similar proportion (45–51%) occurred in July between protein plus FAA and total lipids. The differences in egg size during the spawning season along with variability in batch composition suggests that the female spawning condition is a major factor determining egg quality and early offspring success.

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Acknowledgments

The authors want to thank our colleagues at LOPEL for their cooperation in experimental and field work, R.R. González for his important support in analytical issues, L. Nuñez for her kind cooperation with free amino acid analyses, and R. Veas for his timely collaboration in statistical analyses. We are also grateful to the R/V Kay Kay crew for support during fieldwork and to J. Marileo and C. Valenzuela for help throughout the experimental stage of this study. Financial support and equipment were obtained from the International Foundation of Science (IFS Grant AA 3643-1, to MCK) and FONDECYT Grants 1070502 and 1030819 (LRC and G. Claramunt). MCK was partially supported by the Graduate School of the Universidad de Concepcion, a CONICYT and a COPAS doctoral fellowships and a thesis support grant (AT- 24080047).

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Author notes

  1. S. Vásquez

    Present address: Instituto de Investigación Pesquera (INPESCA), Casilla 350, Talcahuano, Chile

Authors and Affiliations

  1. Laboratorio de Oceanografía Pesquera y Ecología Larval (LOPEL), Departamento de Oceanografía, Universidad de Concepción, Casilla 160-C, Concepción, Chile

    M. C. Krautz, S. Vásquez, L. R. Castro & A. Llanos-Rivera

  2. Programa de Doctorado en Oceanografía, Departamento de Oceanografía, Universidad de Concepción, Casilla 160-C, Concepción, Chile

    M. C. Krautz

  3. Departamento de Oceanografía y Centro de Investigación Oceanográfica en el Pacífico Sur Oriental (COPAS), Universidad de Concepción, Concepción, Chile

    L. R. Castro & S. Pantoja

  4. Departamento de Bioquímica Clínica e Inmunología, Facultad de Farmacia, Universidad de Concepción, Concepción, Chile

    M. González

  5. Unidad de Biotecnología Marina, Facultad de Ciencias Naturales y Oceanográficas, Universidad de Concepción, Concepción, Chile

    A. Llanos-Rivera

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  1. M. C. Krautz
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  4. M. González
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Correspondence to M. C. Krautz.

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Communicated by M. A. Peck.

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Krautz, M.C., Vásquez, S., Castro, L.R. et al. Changes in metabolic substrates during early development in anchoveta Engraulis ringens (Jenyns 1842) in the Humboldt Current. Mar Biol 157, 1137–1149 (2010). https://doi.org/10.1007/s00227-010-1395-7

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