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Culturable actinobacteria isolated from marine sponge Iotrochota sp.

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Abstract

The study describes the diversity of actinobacteria isolated from the marine sponge Iotrochota sp. collected in the South China Sea. Species and natural product diversity of isolates were analyzed, including screening for genes encoding polyketide synthases (PKS) and nonribosomal peptide synthetase (NRPS), and 16S rRNA gene restriction fragment length polymorphism (RFLP). PKS and NRPS sequences were detected in more than half of the isolates and the different “PKS-I–PKS-II–NRPS” combinations in different isolates belonging to the same species indicated a potential natural product diversity and divergent genetic evolution. The phylogenetic analysis based on 16S rRNA gene sequencing showed that the isolates belonged to genera Streptomyces, Cellulosimicrobium, and Nocardiopsis. The majority of the strains tested belonged to the genus Streptomyces and one of them may be a new species. To our knowledge, this is the first report of a bacterium classified as Cellulosimicrobium sp. isolated from a marine sponge.

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Acknowledgments

This research was funded by the Knowledge Innovation Program of the Chinese Academy of Sciences (KZCX2-YW-216, KZCX2-YW-211), and the Research Foundation of Science and Technology Plan Project in Guangdong Province (2006B36501004). We also thank financial supports of Hundred Talent Program of Chinese Academy of Sciences, and Mr. Len Ward for proofreading of this manuscript with valuable suggestions. The experiments comply with the current laws of the country in which the experiments were performed.

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Authors and Affiliations

  1. LMB-CAS, LMMM-GD, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, 510301, People’s Republic of China

    Shumei Jiang, Xiang Li, Wei Sun, Shikun Dai & Lianwu Xie

  2. Floriculture Research Institute of Guangdong Academy of Agricultural Sciences, Guangzhou, 510640, People’s Republic of China

    Long Zhang

  3. LMB-CAS, LMMM-GD, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, 510301, People’s Republic of China

    Yonghong Liu

  4. Department of Biology, Hannam University, 133 Ojungdong, Daedukgu, Daejeon, South Korea

    Kyung Jin Lee

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  1. Shumei Jiang
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  2. Xiang Li
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  3. Long Zhang
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  4. Wei Sun
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  5. Shikun Dai
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  7. Yonghong Liu
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Corresponding author

Correspondence to Xiang Li.

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Communicated by K. Yin.

Key Laboratory of Marine Bio-recourses Sustainable Utilization (LMB-CAS), Guangdong Key Laboratory of Marine Materia Medica (LMMM-GD), South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, People’s Republic of China.

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Jiang, S., Li, X., Zhang, L. et al. Culturable actinobacteria isolated from marine sponge Iotrochota sp.. Mar Biol 153, 945–952 (2008). https://doi.org/10.1007/s00227-007-0866-y

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